State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing 100191, China.
State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing 100191, China.
Phytochemistry. 2019 Jun;162:224-231. doi: 10.1016/j.phytochem.2019.03.012. Epub 2019 Apr 4.
Seven previously undescribed coumarin derivatives, panitins A‒G, an ethoxylated artifact, and 34 known analogues, were isolated from the roots of Murraya paniculata. Their structures were elucidated on the basis of comprehensive analysis of 1D and 2D NMR and HRMS spectroscopic data, and comparison with the data reported in literature. The absolute configurations of undescribed compounds were assigned via comparison of the specific rotation, Mosher's method, exciton chiral method, and comparison of calculated and experimental ECD data. Panitin D, trans-dehydroosthol, and exotimarin I showed potent inhibition against LPS-induced NO production in BV-2 microglial cells with IC values of 19.6 ± 2.3, 12.4 ± 0.9, and 26.9 ± 0.8 μM, respectively.
从芸香科植物两面针的根部分离得到了 7 个以前未描述的香豆素衍生物（panitins A-G）、一个乙氧基化副产物和 34 个已知类似物。基于 1D 和 2D NMR 和高分辨率质谱（HRMS）光谱数据的综合分析，并与文献报道的数据进行比较，确定了它们的结构。通过比较比旋光度、莫舍尔法、外消旋手性法和计算与实验的 ECD 数据，确定了未描述化合物的绝对构型。panitin D、trans-dehydroosthol 和 exotimarin I 对 LPS 诱导的 BV-2 小胶质细胞中 NO 产生具有很强的抑制作用，IC50 值分别为 19.6±2.3、12.4±0.9 和 26.9±0.8 μM。
