Health Sciences Institute, Federal University of Bahia, Salvador, Bahia, Brazil.
Health Sciences Institute, Federal University of Bahia, Salvador, Bahia, Brazil.
Bioresour Technol. 2019 Jul;284:340-348. doi: 10.1016/j.biortech.2019.03.144. Epub 2019 Mar 30.
The objective was to evaluate the effect of reusing Zarrouk's medium on a Spirulina sp. LEB 18 culture by determining kinetic parameters, chemical composition, biofuels, and thermal characterization. Cultivation was performed in a raceway bioreactor for 7 days, the supernatant was reused for four cycles. Culturing the microalga in the reused medium resulted in a cellular yield of 2.30 g L (control) and 2.04, 1.89, 1.73, and 1.15 g L for four cycles with no influence on cell growth and productivity. Biomass with high contents of carbohydrates (58.00%, 3rd cycle), phycocyanin (2.47 mg mL, 1st cycle), and saturated fatty acids (60.13%, 4th cycle) were obtained with an increase in the profiles of C16:0 (45.85%) and C18:2n6 (47.40%) in the 1st cycle. The reuse of Zarrouk's medium allowed obtaining biomass with reduced cost and differentiated characteristics, allowing the exploration of commercially important biomolecules by the completion of up to four cycles.
本研究旨在通过测定动力学参数、化学成分、生物燃料和热特性来评估重复使用 Zarrouk 培养基对螺旋藻 LEB 18 培养的影响。在跑道式生物反应器中进行了 7 天的培养，将上清液重复使用了 4 个周期。在重复使用的培养基中培养微藻，细胞产率为 2.30 g/L（对照），第 4 个周期分别为 2.04、1.89、1.73 和 1.15 g/L，对细胞生长和生产力没有影响。第 1 个周期获得了具有高碳水化合物含量（58.00%）、藻蓝蛋白（2.47 mg/mL，第 1 个周期）和饱和脂肪酸（60.13%，第 4 个周期）的生物质，同时 C16:0（45.85%）和 C18:2n6（47.40%）的比例增加。重复使用 Zarrouk 培养基可以获得成本更低、特性不同的生物质，允许通过完成多达 4 个周期来探索具有商业重要性的生物分子。
