Dejsungkranont Monchai, Chisti Yusuf, Sirisansaneeyakul Sarote
Department of Biotechnology, Faculty of Agro-Industry, Kasetsart University, Bangkok, 10900, Thailand.
School of Engineering, Massey University, Private Bag 11 222, Palmerston North, New Zealand.
Bioprocess Biosyst Eng. 2017 Aug;40(8):1173-1188. doi: 10.1007/s00449-017-1778-y. Epub 2017 May 11.
The key factors influencing the production of C-phycocyanin (C-PC) and extracellular polymeric substances (EPS) by photoautotrophic culture of Arthrospira sp. were optimized using Taguchi method. Six factors were varied at either three or two levels as follows: light intensity at three levels; three initial culture pHs; two species of Arthrospira; three concentrations of Zarrouk's medium; three rates of aeration of the culture with air mixed with 2% v/v carbon dioxide; and two incubation temperatures. All cultures ran for 14 days. The optimal conditions for the production of C-PC and EPS were different. For both products, the best cyanobacterium proved to be Arthrospira maxima IFRPD1183. The production of C-PC was maximized with the following conditions: a light intensity of 68 µmol photons m s (a diurnal cycle of 16-h photoperiod and 8-h dark period), an initial pH of 10, the full strength (100%) Zarrouk's culture medium, an aeration rate of 0.6 vvm (air mixed with 2% v/v CO) and a culture temperature of 30 °C. The concentration of Zarrouk's medium was the most important factor influencing the final concentration of C-PC. The optimal conditions for maximal production of EPS were as follows: a light intensity of 203 µmol photons m s with the earlier specified light-dark cycle; an initial pH of 9.5; a 50% strength of Zarrouk's medium; an aeration rate of 0.2 vvm (air mixed with 2% v/v CO); and a temperature of 35 °C. Production of C-PC and EPS in raceway ponds is discussed.
利用田口方法优化了影响钝顶螺旋藻光合自养培养生产C-藻蓝蛋白（C-PC）和胞外聚合物（EPS）的关键因素。六个因素在三个或两个水平上变化，具体如下：光照强度设三个水平；三种初始培养pH值；两种钝顶螺旋藻；三种Zarrouk培养基浓度；三种用2%（v/v）二氧化碳混合空气的培养通气速率；以及两种培养温度。所有培养均持续14天。C-PC和EPS生产的最佳条件不同。对于这两种产物，最佳蓝藻均为极大螺旋藻IFRPD1183。在以下条件下C-PC产量最大化：光照强度为68 μmol光子·m⁻²·s⁻¹（16小时光周期和8小时暗周期的昼夜循环），初始pH值为10，全强度（100%）Zarrouk培养基，通气速率为0.6 vvm（2%（v/v）CO₂混合空气），培养温度为30℃。Zarrouk培养基浓度是影响C-PC最终浓度的最重要因素。EPS最大产量的最佳条件如下：光照强度为203 μmol光子·m⁻²·s⁻¹，采用上述光暗循环；初始pH值为9.5；50%强度的Zarrouk培养基；通气速率为0.2 vvm（2%（v/v）CO₂混合空气）；温度为35℃。文中还讨论了跑道池中C-PC和EPS的生产情况。
