Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of School of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, China.
College of Pharmaceutical Science, Southwest University, Chongqing, 400716, China.
Biosens Bioelectron. 2019 Jun 1;134:29-35. doi: 10.1016/j.bios.2019.03.058. Epub 2019 Mar 29.
Metal-organic gels (MOGs), a kind of soft materials, were reported as a brand new category of novel solid coreactants for enhancing electrochemiluminescence (ECL) of Ru (bpy) and derivatives. In this work, silver-based MOGs (Ag-MOGs) prepared by a one-step mixing and heating approach were demonstrated as novel efficient solid-state coreactants which differ from the previous research on ECL coreactant nanomaterials. It was discussed that the possible enhancement mechanism was due to the amino-rich groups and the electro-catalytic effect of Ag-MOGs, endowing them promising coreactants for the enhancement of Ru (bpy) ECL. Interestingly, Ag-MOGs can also enhance the ECL signal of Ru (phen), a derivative of Ru (bpy) that can specifically insert into double strand DNA, further manifesting the as-prepared Ag-MOGs can be served as ideal coreactant materials. Based on the Ru (phen)/Ag-MOGs system, a label-free signal-on ECL biosensor for detecting of target DNA was constructed to illustrate the potential application of Ag-MOGs. This research presents an effective coreactant for Ru luminophore-based ECL systems, which not only broadens the potential application of MOGs, but also offers a unique insight into the coreactant materials.
金属有机凝胶（MOG）是一种软物质，被报道为增强钌（bpy）及其衍生物电化学发光（ECL）的新型固态反应试剂的全新类别。在这项工作中，通过一步混合和加热方法制备的基于银的 MOG（Ag-MOG）被证明是新型高效固态反应试剂，与之前关于 ECL 反应试剂纳米材料的研究不同。讨论了可能的增强机制是由于 Ag-MOG 中的富含氨基的基团和电催化作用，赋予它们作为增强 Ru（bpy）ECL 的有前途的反应试剂。有趣的是，Ag-MOG 还可以增强 Ru（phen）的 ECL 信号，Ru（phen）是 Ru（bpy）的衍生物，可以特异性插入双链 DNA，进一步表明所制备的 Ag-MOG 可以用作理想的反应试剂材料。基于 Ru（phen）/Ag-MOG 体系，构建了用于检测靶 DNA 的无标记信号开启 ECL 生物传感器，以说明 Ag-MOG 的潜在应用。这项研究为基于 Ru 发光体的 ECL 系统提供了一种有效的反应试剂，不仅拓宽了 MOG 的潜在应用，而且为反应试剂材料提供了独特的见解。
