Zhou Xing, He Jing, Wang Lingling, Wang Yang, Du Guocheng, Kang Zhen
The Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, P.R. China.
The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, P.R. China.
J Microbiol Biotechnol. 2019 May 28;29(5):758-764. doi: 10.4014/jmb.1812.12049.
β-Glucan is a chief structural polymer in the cell wall of yeast. β-Glucan has attracted intensive attention because of its wide applications in health protection and cosmetic areas. In the present study, the β-glucan biosynthesis pathway in was engineered to enhance β-glucan accumulation. A newly identified bacterial β-1, 6-glucan synthase GsmA from was expressed, and increased β-glucan content by 43%. In addition, other pathway enzymes were investigated to direct more metabolic flux towards the building of β-glucan chains. We found that overexpression of Pgm2 (phosphoglucomutase) and Rho1 (a GTPase for activating glucan synthesis) significantly increased β-glucan accumulation. After further optimization of culture conditions, the β-glucan content was increased by 53.1%. This study provides a new approach to enhance β-glucan biosynthesis in .
β-葡聚糖是酵母细胞壁中的主要结构聚合物。由于其在健康保护和化妆品领域的广泛应用，β-葡聚糖已引起广泛关注。在本研究中，对[具体对象]的β-葡聚糖生物合成途径进行了改造，以提高β-葡聚糖的积累。表达了一种新鉴定的来自[具体来源]的细菌β-1,6-葡聚糖合酶GsmA，使β-葡聚糖含量增加了43%。此外，还研究了其他途径酶，以引导更多的代谢通量用于构建β-葡聚糖链。我们发现过表达Pgm2（磷酸葡萄糖变位酶）和Rho1（一种激活葡聚糖合成的GTP酶）可显著增加β-葡聚糖的积累。在进一步优化培养条件后，β-葡聚糖含量增加了53.1%。本研究为提高[具体对象]中β-葡聚糖的生物合成提供了一种新方法。
