Cajot J F, Sordat B, Bachmann F
J Natl Cancer Inst. 1986 Nov;77(5):1099-107.
The characterization and quantitation of plasminogen activators (PAs) expressed by human colon carcinoma cell lines and primary colon carcinomas inoculated into nude outbred (nu/nu) mice, either as subcutaneous or gut-implanted (GI) xenografts, were discussed. The two colon carcinoma cell lines used in this study, Col 112 (moderately differentiated) and Col 115 (poorly differentiated), differ in their PA expression, the former being a urinary-type PA and the latter being a tissue-type PA producer. Both cell lines demonstrate a positive correlation between tumor invasiveness and measured PA activity; subcutaneous xenografts growing as noninvasive pseudobenign tumor masses were associated with low levels of PA activity, whereas GI xenografts exhibiting invasive growth expressed higher PA activity. Furthermore, coinoculation of Col 115 tumor cells sc and GI in the same host induced high levels of PA activity in subcutaneous xenografts, suggesting a stimulatory effect of the GI xenograft on subcutaneous xenograft PA expression. Also, purified murine plasminogen was demonstrated to represent an efficient substrate for tumor-secreted human PA.
讨论了人结肠癌细胞系和接种到裸系远交（nu/nu）小鼠体内的原发性结肠癌（以皮下或肠道植入（GI）异种移植物形式）所表达的纤溶酶原激活剂（PA）的特性和定量。本研究中使用的两种结肠癌细胞系，Col 112（中度分化）和Col 115（低分化），在PA表达上有所不同，前者产生尿激酶型PA，后者产生组织型PA。两种细胞系均显示肿瘤侵袭性与测得的PA活性之间呈正相关；以非侵袭性假良性肿瘤块生长的皮下异种移植物与低水平的PA活性相关，而表现出侵袭性生长的GI异种移植物表达较高的PA活性。此外，在同一宿主中同时皮下和肠道植入Col 115肿瘤细胞会在皮下异种移植物中诱导高水平的PA活性，这表明GI异种移植物对皮下异种移植物PA表达有刺激作用。而且，已证明纯化的小鼠纤溶酶原是肿瘤分泌的人PA的有效底物。
