Shah V, Kumar S, Zirvi K A
Department of Biochemistry and Molecular Biology, UMD-New Jersey Medical School, Newark 07103-2714.
In Vivo. 1994 May-Jun;8(3):321-6.
Metastatic spread of tumor cells depends upon intravasation of malignant cells from the primary site and extravasation into the distant organs following remodeling of the basement membrane. We have investigated the metastatic potential of five tumorigenic human colon carcinoma cell lines, LS 174T, SW 620, WiDr, SW 480 and Caco-2 using intrasplenic injection in nude mice. LS 174T is most aggressive causing liver metastasis in all animals within 6 weeks. SW 620 and WiDr produced liver metastasis in 70% and 30% of the animals but after a period of 12 weeks whereas SW 480 and Caco-2 were not metastatic. LS 174T exhibited high cell-associated urokinase-type plasminogen activator (u-PA) and high secreted u-PA and tissue plasminogen activator (t-PA) levels. WiDr, SW 480 and Caco-2 had essentially similar low levels of cell associated u-PA but WiDr had higher secreted u-PA levels as comprated to the SW 480 and Caco-2 cells. The level of secreted MMP-2 (72 kDa gelatinase) was highest in the most metastatic cell line, LS 174T, and lower in other less metastatic ones. These data show that metastatic behavior of human colon tumor cells correlates with the enhanced secretion of plasminogen activators and MMP-2 by these cells.
肿瘤细胞的转移扩散取决于恶性细胞从原发部位侵入血管以及在基底膜重塑后渗入远处器官。我们通过在裸鼠脾脏内注射，研究了五种致瘤性人结肠癌细胞系LS 174T、SW 620、WiDr、SW 480和Caco-2的转移潜能。LS 174T最具侵袭性，在6周内使所有动物发生肝转移。SW 620和WiDr分别在70%和30%的动物中发生肝转移，但在12周后，而SW 480和Caco-2不具有转移性。LS 174T表现出高细胞相关尿激酶型纤溶酶原激活剂（u-PA）以及高分泌型u-PA和组织纤溶酶原激活剂（t-PA）水平。WiDr、SW 480和Caco-2具有基本相似的低水平细胞相关u-PA，但与SW 480和Caco-2细胞相比，WiDr具有更高的分泌型u-PA水平。分泌型MMP-2（72 kDa明胶酶）水平在转移性最强的细胞系LS 174T中最高，在其他转移性较弱的细胞系中较低。这些数据表明，人结肠肿瘤细胞的转移行为与这些细胞中纤溶酶原激活剂和MMP-2分泌的增强相关。
