MOE International Joint Collaborative Research Laboratory for Animal Health and Food Safety, Institute of Immunology and College of Veterianry Medicine, Nanjing Agricultural University, Nanjing, China.
MOA Key Laboratory of Animal Virology, Department of Veterinary Medicine, Zhejiang University, Hangzhou, China.
Vet Microbiol. 2019 Apr;231:238-245. doi: 10.1016/j.vetmic.2019.03.012. Epub 2019 Mar 14.
Circular RNAs (circRNAs) play critical roles in various diseases. However, whether and how circular RNA regulates influenza A virus (IAV) infection is unknown. Here, we studied the role of circular RNA GATA Zinc Finger Domain Containing 2A (circ-GATAD2A) in the replication of IAV H1N1 in A549 cells. Circ-GATAD2A was formed upon H1N1 infection. Knockdown of circ-GATAD2A in A549 cells enhanced autophagy and inhibited H1N1 replication. By contrast, overexpression of circ-GATAD2A impaired autophagy and promoted H1N1 replication. Similarly, knockout of vacuolar protein sorting 34 (VPS34) blocked autophagy and increased H1N1 replication. However, the expression of circ-GATAD2A could not further enhance H1N1 replication in VPS34 knockout cells. Collectively, these data indicated that circ-GATAD2A promotes the replication of H1N1 by inhibiting autophagy.
环状 RNA（circRNAs）在各种疾病中发挥着关键作用。然而，环状 RNA 是否以及如何调节甲型流感病毒（IAV）感染尚不清楚。在这里，我们研究了环状 RNA GATA 锌指结构域包含 2A（circ-GATAD2A）在 A549 细胞中甲型 H1N1 流感病毒复制中的作用。在 H1N1 感染后形成了 circ-GATAD2A。在 A549 细胞中敲低 circ-GATAD2A 增强了自噬并抑制了 H1N1 复制。相比之下，circ-GATAD2A 的过表达会损害自噬并促进 H1N1 复制。同样，液泡蛋白分选 34（VPS34）的敲除会阻断自噬并增加 H1N1 复制。然而，circ-GATAD2A 的表达不能进一步增强 VPS34 敲除细胞中的 H1N1 复制。总之，这些数据表明，circ-GATAD2A 通过抑制自噬促进了 H1N1 的复制。
