Anucherngchai Sothorn, Chontananarth Thapana, Tejangkura Thanawan, Chai Jong-Yil
1Applied Parasitology Research Laboratory, Department of Biology, Faculty of Science, Srinakharinwirot University, Bangkok, Thailand.
2Center of Excellence in Animal, Plant and Parasitic Biotechnology, Srinakharinwirot University, Bangkok, Thailand.
J Parasit Dis. 2019 Mar;43(1):66-74. doi: 10.1007/s12639-018-1057-0. Epub 2018 Nov 20.
is known as a significant intestinal trematode in various species of animals and humans. It presents complexities in terms of both the morphological and molecular biological data. This is the first study of the application of gene () as a target for studying the phylogeny and designing species-specific primer of . Adult trematodes were harvested from experimentally infected hamsters at 18 days of post-infection. Each worm was identified based on their morphological appearance. The novel primers were designed from other species to initially amplify region in . All sequence data of in five provinces of Central Thailand were used as the target for designing the species-specific primer for . The results revealed that gene can separate into two sister groups by geographical distribution, comprising the eastern and western area groups. Moreover, it also separates from other species, including two sibling species; and . In addition, we developed the high performance species-specific primer of . It can detect DNA from a single egg, as well as cercaria, metacercaria and adult stages of this trematode with no cross-reactions to other trematodes and their hosts. Therefore, this research is a positive initial step for the future study of . The future studies based on this gene should be continued with all species in revolutum complex to overcome the problems of systemic classification that arise in this complex group.
在各种动物和人类中，它被认为是一种重要的肠道吸虫。在形态学和分子生物学数据方面都存在复杂性。这是首次将基因（）作为研究系统发育和设计物种特异性引物的靶点进行的研究。在感染后18天从实验感染的仓鼠中采集成年吸虫。每条虫根据其形态外观进行鉴定。从其他物种设计了新的引物，以初步扩增中的区域。泰国中部五个省份的所有序列数据被用作设计物种特异性引物的靶点。结果表明，基因可以根据地理分布将分为两个姐妹群，包括东部和西部地区群。此外，它还将与其他物种区分开来，包括两个近缘物种；和。此外，我们开发了高性能的物种特异性引物。它可以检测单个虫卵以及该吸虫的尾蚴、囊蚴和成虫阶段的DNA，且与其他吸虫及其宿主无交叉反应。因此，这项研究是未来研究的一个积极的初步步骤。基于该基因的未来研究应继续针对复殖吸虫复合体中的所有物种，以克服该复杂群体中出现的系统分类问题。
