Kato Hirotomo, Gomez Eduardo A, Martini-Robles Luiggi, Muzzio Jenny, Velez Lenin, Calvopiña Manuel, Romero-Alvarez Daniel, Mimori Tatsuyuki, Uezato Hiroshi, Hashiguchi Yoshihisa
Division of Medical Zoology, Department of Infection and Immunity, Jichi Medical University, Tochigi, Japan.
Laboratory of Parasitology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Hokkaido, Japan.
PLoS Negl Trop Dis. 2016 Jul 13;10(7):e0004844. doi: 10.1371/journal.pntd.0004844. eCollection 2016 Jul.
A countrywide epidemiological study was performed to elucidate the current geographic distribution of causative species of cutaneous leishmaniasis (CL) in Ecuador by using FTA card-spotted samples and smear slides as DNA sources. Putative Leishmania in 165 samples collected from patients with CL in 16 provinces of Ecuador were examined at the species level based on the cytochrome b gene sequence analysis. Of these, 125 samples were successfully identified as Leishmania (Viannia) guyanensis, L. (V.) braziliensis, L. (V.) naiffi, L. (V.) lainsoni, and L. (Leishmania) mexicana. Two dominant species, L. (V.) guyanensis and L. (V.) braziliensis, were widely distributed in Pacific coast subtropical and Amazonian tropical areas, respectively. Recently reported L. (V.) naiffi and L. (V.) lainsoni were identified in Amazonian areas, and L. (L.) mexicana was identified in an Andean highland area. Importantly, the present study demonstrated that cases of L. (V.) braziliensis infection are increasing in Pacific coast areas.
通过使用FTA卡斑点样本和涂片玻片作为DNA来源，开展了一项全国性的流行病学研究，以阐明厄瓜多尔皮肤利什曼病（CL）病原体的当前地理分布情况。基于细胞色素b基因序列分析，对从厄瓜多尔16个省份的CL患者中采集的165份样本中的推定利什曼原虫进行了种属水平的检测。其中，125份样本被成功鉴定为圭亚那利什曼原虫（Viannia亚属）、巴西利什曼原虫（Viannia亚属）、奈菲利什曼原虫（Viannia亚属）、兰氏利什曼原虫（Viannia亚属）和墨西哥利什曼原虫（Leishmania亚属）。两种优势种，即圭亚那利什曼原虫（Viannia亚属）和巴西利什曼原虫（Viannia亚属），分别广泛分布于太平洋沿岸亚热带地区和亚马逊热带地区。最近报道的奈菲利什曼原虫（Viannia亚属）和兰氏利什曼原虫（Viannia亚属）在亚马逊地区被鉴定出来，而墨西哥利什曼原虫（Leishmania亚属）在安第斯高地地区被鉴定出来。重要的是，本研究表明，太平洋沿岸地区巴西利什曼原虫（Viannia亚属）感染病例正在增加。
