Baig Salma, Azizan Ainnul Hamidah Syahadah, Raghavendran Hanumantha Rao Balaji, Natarajan Elango, Naveen Sangeetha, Murali Malliga Raman, Nam Hui Yin, Kamarul Tunku
Tissue Engineering Group (TEG), National Orthopaedic Centre of Excellence in Research and Learning (NOCERAL), Department of Orthopaedic Surgery, Faculty of Medicine, University of Malaya, Lembah Pantai, 50603 Kuala Lumpur, Malaysia.
Research Centre for Crystalline Materials, School of Science and Technology, Sunway University, 47500 Selangor, Malaysia.
Stem Cells Int. 2019 Feb 26;2019:5142518. doi: 10.1155/2019/5142518. eCollection 2019.
We have determined the protective effects of (TS) extract and nanoparticle-loaded TS on hydrogen peroxide-induced cell death of mesenchymal stromal cells (MSCs) . Gas chromatography-mass spectroscopy confirmed the spectrum of active components in the extract. Out of the three different extracts, the hexane extract showed significant free radical scavenging activity. Treatment of MSCs with HO (hydrogen peroxide) significantly increased intracellular cell death; however, pretreatment with TS extract and nanoparticle-loaded TS (200 g/ml) suppressed HO-induced elevation of Cyt-c and MMP13 and increased the survival rates of MSCs. HO-induced (0.1 mM) changes in cytokines were attenuated in the extract and nanoparticles by pretreatment and cotreatment at two time points ( < 0.05). HO increased cell apoptosis. In contrast, treatment with nanoparticle-loaded TS suppressed the percentage of apoptosis considerably ( < 0.05). Therefore, TS may be considered as a potential candidate for enhancing the effectiveness of MSC transplantation in cell therapy.
我们已经确定了（TS）提取物和负载纳米颗粒的TS对过氧化氢诱导的间充质基质细胞（MSCs）细胞死亡的保护作用。气相色谱 - 质谱法证实了提取物中活性成分的光谱。在三种不同的提取物中，己烷提取物表现出显著的自由基清除活性。用过氧化氢（HO）处理MSCs显著增加细胞内细胞死亡；然而，用TS提取物和负载纳米颗粒的TS（200μg/ml）预处理可抑制HO诱导的细胞色素c（Cyt-c）和基质金属蛋白酶13（MMP13）升高，并提高MSCs的存活率。在两个时间点通过预处理和联合处理，提取物和纳米颗粒减弱了HO诱导的（0.1mM）细胞因子变化（P<0.05）。HO增加细胞凋亡。相比之下，用负载纳米颗粒的TS处理可显著抑制凋亡百分比（P<0.05）。因此，TS可被视为提高MSCs移植在细胞治疗中有效性的潜在候选物。
