Feng Tieshan, Lin Ping, Gong Jiao, Cheng Dong, Yang Xi, Zhang Quan, Cheng Tingcai
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China.
Chongqing Engineering and Technology Research Center for Novel Silk Materials, Southwest University, Chongqing 400715, China.
Int J Genomics. 2019 Mar 5;2019:7859121. doi: 10.1155/2019/7859121. eCollection 2019.
is a pathogen of ; it can cause bacterial septicemia in silkworm. One of the components of the parasporal crystal toxin of , -toxin, plays an important role in the process of infection in silkworm. In this study, we investigated the immune response of silkworm induced by -toxin by using RNA-seq. We compared the changes in gene expression in the midgut, fatbody, and hemocytes of silkworm and in the embryonic cell line (BmE) after treatment with -toxin and identified 952 differentially expressed genes and 353 differentially expressed long noncoding RNAs (lncRNAs). These regulated genes in different tissues were found to be enriched in different pathways. The upregulated genes in the midgut were mainly involved in peptidoglycan catabolic process and tyrosine kinase signaling pathway, whereas the downregulated genes were mainly involved in chitin metabolic pathways. The upregulated genes in fatbody were also involved in peptidoglycan catabolic process, but they were for a different peptidoglycan subtype. Further, genes encoding cecropins were enriched in the fatbody. The downregulated genes were mainly involved in the metabolic pathways of fundamental substances such as cellular protein metabolic process and nucleobase-containing compound metabolic process. These results suggest that -toxin can induce various immune responses in silkworm, and further studies are warranted to understand the mechanism of -toxin action in silkworm. Further, lncRNAs and differentially expressed genes were correlated using coexpression network analysis. Our findings revealed potential candidate genes and lncRNAs that might play important physiological functions in the immune response to -toxins in silkworm.
是……的病原体；它可导致家蚕细菌性败血症。……伴胞晶体毒素的成分之一，即……毒素，在家蚕感染过程中起重要作用。在本研究中，我们通过RNA测序研究了……毒素诱导的家蚕免疫反应。我们比较了……毒素处理后家蚕中肠、脂肪体和血细胞以及胚胎细胞系（BmE）中基因表达的变化，鉴定出952个差异表达基因和353个差异表达长链非编码RNA（lncRNA）。发现不同组织中的这些调控基因富集于不同的通路。中肠中上调的基因主要参与肽聚糖分解代谢过程和酪氨酸激酶信号通路，而下调的基因主要参与几丁质代谢通路。脂肪体中上调的基因也参与肽聚糖分解代谢过程，但针对的是不同的肽聚糖亚型。此外，编码杀菌肽的基因在脂肪体中富集。下调的基因主要参与细胞蛋白质代谢过程和含核碱基化合物代谢过程等基本物质的代谢通路。这些结果表明，……毒素可诱导家蚕产生多种免疫反应，有必要进一步研究以了解……毒素在家蚕中的作用机制。此外，使用共表达网络分析将lncRNA与差异表达基因进行了关联。我们的研究结果揭示了可能在家蚕对……毒素的免疫反应中发挥重要生理功能的潜在候选基因和lncRNA。
