College of Resources and Environment, Southwest University, Chongqing, China.
Ministry of Education Key Laboratory for Ecology of Tropical Islands, College of Life Science, Hainan Normal University, China.
Mol Plant Microbe Interact. 2019 Sep;32(9):1063-1066. doi: 10.1094/MPMI-01-19-0004-LE. Epub 2019 Jul 29.
The pRC system is an efficient tool for genetic studies in , ensuring direct insertion of foreign gene elements into chromosome downstream of . This system is designed for double recombination across and the downstream region, which considerably simplifies genetic studies of complementation, overexpression, pathogenicity, and in-vivo promoter activity assays with monocopy in , one of the most destructive plant-pathogenic bacteria worldwide. is extremely heterogeneous and is currently referred to as a species complex (RSSC). The gene is greatly conserved, but its downstream regions are mostly different in the RSSC, which limits the application of the current pRC plasmid in the RSSC. We compared all existing 132 genome sequences in a precise genomic downstream region and confirmed that the pRC system is appropriate for application of chromosomal integration in all RSSC strains but needs respective reconstruction on current pRC plasmids, since downstream regions are greatly variable in the RSSC. RSSC strains can be grouped according to identical downstream regions. This grouping provides valuable information for gene insertion in this chromosomal region, as it facilitates universal application of the pRC system in RSSC strains.
pRC 系统是一种在 中进行遗传研究的有效工具，可确保将外源基因元件直接插入 染色体下游的 位置。该系统旨在实现 与下游区域的双交换，这极大地简化了对 的互补、过表达、致病性以及单拷贝体内启动子活性测定等方面的遗传研究， 是全球最具破坏性的植物病原菌之一。 非常多样化，目前被称为 种复合体 (RSSC)。 基因高度保守，但 RSSC 中的下游区域大多不同，这限制了当前 pRC 质粒在 RSSC 中的应用。我们在精确的基因组 下游区域比较了所有现有的 132 个 基因组序列，并证实 pRC 系统适用于 RSSC 菌株中的染色体整合，但需要对当前的 pRC 质粒进行相应的重建，因为 RSSC 中的 下游区域变化很大。RSSC 菌株可以根据相同的 下游区域进行分组。这种分组为该染色体区域的基因插入提供了有价值的信息，因为它促进了 pRC 系统在 RSSC 菌株中的通用应用。
