Smith D B, Davern K M, Board P G, Tiu W U, Garcia E G, Mitchell G F
Proc Natl Acad Sci U S A. 1986 Nov;83(22):8703-7. doi: 10.1073/pnas.83.22.8703.
Mice of the inbred strain 129/J bred at this Institute (WEHI 129/J) are relatively resistant to chronic infection with the parasitic helminth Schistosoma japonicum. In contrast to more permissive mouse strains such as BALB/c, the WEHI 129/J mice are high responders to a Mr 26,000 adult worm antigen designated Sj26. Cloned cDNAs corresponding to Sj26 have been identified in a S. japonicum phage lambda gt11 amp3 expression library, and their nucleotide sequences have been deduced. The predicted amino acid sequence of the antigen specified by these cDNAs shows striking homology with class mu isozymes of mammalian glutathione S-transferases (RX:glutathione R-transferase, EC 2.5.1.18). Extracts of adult worms contain glutathione S-transferase activity, and affinity chromatography of enzyme activity on glutathione columns leads to the purification of a Mr 26,000 molecule that comigrates with Sj26. Although vaccination studies in mice with a beta-galactosidase-Sj26 fusion protein from Escherichia coli are encouraging, more immunogenic preparations of the antigen are likely to be required to establish the utility of Sj26 as a model vaccine.
在本研究所培育的近交系129/J小鼠（WEHI 129/J）对日本血吸虫这种寄生性蠕虫的慢性感染具有相对抗性。与BALB/c等更易感的小鼠品系不同，WEHI 129/J小鼠对一种名为Sj26的26,000道尔顿成虫抗原反应强烈。在日本血吸虫噬菌体λgt11 amp3表达文库中已鉴定出与Sj26对应的克隆cDNA，并推导了它们的核苷酸序列。这些cDNA所指定抗原的预测氨基酸序列与哺乳动物谷胱甘肽S-转移酶的μ类同工酶（RX：谷胱甘肽R-转移酶，EC 2.5.1.18）具有显著同源性。成虫提取物含有谷胱甘肽S-转移酶活性，通过谷胱甘肽柱对酶活性进行亲和层析可纯化出一种与Sj26共迁移的26,000道尔顿分子。尽管用来自大肠杆菌的β-半乳糖苷酶-Sj26融合蛋白对小鼠进行疫苗接种研究令人鼓舞，但可能需要更具免疫原性的抗体制剂来确定Sj26作为模型疫苗的效用。
