Delta-like 蛋白 3 在小细胞肺癌中的流行情况及 DLL3（SP347）检测的特点。
Delta-like Protein 3 Prevalence in Small Cell Lung Cancer and DLL3 (SP347) Assay Characteristics.
机构信息
From the Department of Companion Diagnostics, Ventana Medical Systems, Tucson, Arizona.
出版信息
Arch Pathol Lab Med. 2019 Nov;143(11):1373-1377. doi: 10.5858/arpa.2018-0497-OA. Epub 2019 Apr 8.
CONTEXT.—: Delta-like protein 3 (DLL3) is a protein that is implicated in the Notch pathway.
OBJECTIVE.—: To present data on DLL3 prevalence in small cell lung cancer and staining characteristics of the VENTANA DLL3 (SP347) Assay. In addition, the assay's immunoreactivity with other neoplastic and nonneoplastic tissues is outlined.
DESIGN.—: Individual formalin-fixed, paraffin-embedded specimens of small cell lung cancer and tissue microarrays comprising neoplastic and nonneoplastic tissues were procured. Sections were cut and stained with DLL3 (SP347) assay. The slides were examined to determine prevalence, staining characteristics, and immunoreactivity.
RESULTS.—: Cytoplasmic and/or membranous staining was observed in 1040 of 1362 specimens of small cell lung cancer (76.4%). Homogenous and/or heterogeneous and partial and/or circumferential granular staining with varied intensities was noted. Immunoreactivity was also observed in other neoplastic and nonneoplastic tissues.
CONCLUSIONS.—: Our study findings provided the profile of DLL3 staining characteristics that can be used for determining the level of DLL3 expression in small cell lung cancer.
背景
Delta 样蛋白 3（DLL3）是一种与 Notch 通路相关的蛋白。
目的
介绍 DLL3 在小细胞肺癌中的表达情况以及 VENTANA DLL3（SP347）检测的染色特征。此外，还概述了该检测对其他肿瘤性和非肿瘤性组织的免疫反应性。
设计
收集了小细胞肺癌的福尔马林固定、石蜡包埋标本的单个样本和包含肿瘤性和非肿瘤性组织的组织微阵列。制作切片并使用 DLL3（SP347）检测进行染色。观察切片以确定 DLL3 的表达率、染色特征和免疫反应性。
结果
在 1362 份小细胞肺癌标本中的 1040 份（76.4%）中观察到细胞质和/或膜染色。存在均匀和/或不均匀、部分和/或环状、不同强度的颗粒状染色。在其他肿瘤性和非肿瘤性组织中也观察到免疫反应性。
结论
我们的研究结果提供了 DLL3 染色特征的概况，可用于确定小细胞肺癌中 DLL3 的表达水平。
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