• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

工程化外泌体可恢复子宫体子宫内膜癌中miR-508-5p的表达，并通过靶向DLL3减少肿瘤进展和转移。

Engineered exosomes restore miR-508-5p expression in uterine corpus endometrial carcinoma and reduce tumor progression and metastasis by targeting DLL3.

作者信息

Li Yue-Ying, Liu Hui, Feng Jia-Lu, Tian Wen-Yan, Du Juan, Zhang Li-Ping

机构信息

Department of Gynecology and Obstetrics, Tianjin Medical University General Hospital, Tianjin, China.

Tianjin Key Laboratory of Female Reproductive Health and Eugenic, Tianjin Medical University General Hospital, Tianjin, China.

出版信息

Front Oncol. 2025 Feb 24;15:1532564. doi: 10.3389/fonc.2025.1532564. eCollection 2025.

DOI:10.3389/fonc.2025.1532564
PMID:40066092
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11891050/
Abstract

INTRODUCTION

Endometrial cancer (EC) is a growing global health concern. Understanding the molecular mechanisms driving EC is crucial for developing effective diagnostic and therapeutic strategies. This study investigates the roles of DLL3 and miR-508-5p in EC progression and explores a therapeutic approach using engineered exosomes to modulate their expression.

METHODS

TCGA data were analyzed, and experiments were performed to assess DLL3 and miR-508-5p function, and bioinformatics was used to confirm their interaction. Mesenchymal stem cells (MSCs) were engineered to produce miR-508-5p-overexpressing exosomes, and their therapeutic effects were tested in mouse models.

RESULTS

Elevated DLL3 and downregulated miR-508-5p were observed in EC and correlated with poor outcomes. miR-508-5p directly targets DLL3. Engineered exosomes restored miR-508-5p, inhibited DLL3, and reduced tumor growth and metastasis in mouse models.

DISCUSSION

The findings highlight the roles of DLL3 and miR-508-5p in EC. Targeting the miR-508-5p/DLL3 axis via exosome-mediated delivery represents a promising therapeutic strategy for EC.

摘要

引言

子宫内膜癌（EC）是一个日益引起全球健康关注的问题。了解驱动EC的分子机制对于制定有效的诊断和治疗策略至关重要。本研究调查了DLL3和miR-508-5p在EC进展中的作用，并探索了一种使用工程化外泌体调节其表达的治疗方法。

方法

分析了TCGA数据，并进行实验评估DLL3和miR-508-5p的功能，还利用生物信息学证实它们的相互作用。对间充质干细胞（MSC）进行工程改造以产生过表达miR-508-5p的外泌体，并在小鼠模型中测试其治疗效果。

结果

在EC中观察到DLL3升高和miR-508-5p下调，且与不良预后相关。miR-508-5p直接靶向DLL3。工程化外泌体恢复了miR-508-5p，抑制了DLL3，并减少了小鼠模型中的肿瘤生长和转移。

讨论

这些发现突出了DLL3和miR-508-5p在EC中的作用。通过外泌体介导的递送靶向miR-508-5p/DLL3轴代表了一种有前景的EC治疗策略。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f738/11891050/5d7099297965/fonc-15-1532564-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f738/11891050/a724b966029e/fonc-15-1532564-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f738/11891050/877bba22c256/fonc-15-1532564-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f738/11891050/3d0f81724521/fonc-15-1532564-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f738/11891050/57ff6b72f7b2/fonc-15-1532564-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f738/11891050/54769ac384bb/fonc-15-1532564-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f738/11891050/e56c459fc34e/fonc-15-1532564-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f738/11891050/b9a5d5363a84/fonc-15-1532564-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f738/11891050/5d7099297965/fonc-15-1532564-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f738/11891050/a724b966029e/fonc-15-1532564-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f738/11891050/877bba22c256/fonc-15-1532564-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f738/11891050/3d0f81724521/fonc-15-1532564-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f738/11891050/57ff6b72f7b2/fonc-15-1532564-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f738/11891050/54769ac384bb/fonc-15-1532564-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f738/11891050/e56c459fc34e/fonc-15-1532564-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f738/11891050/b9a5d5363a84/fonc-15-1532564-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f738/11891050/5d7099297965/fonc-15-1532564-g008.jpg

相似文献

1
Engineered exosomes restore miR-508-5p expression in uterine corpus endometrial carcinoma and reduce tumor progression and metastasis by targeting DLL3.工程化外泌体可恢复子宫体子宫内膜癌中miR-508-5p的表达，并通过靶向DLL3减少肿瘤进展和转移。
Front Oncol. 2025 Feb 24;15:1532564. doi: 10.3389/fonc.2025.1532564. eCollection 2025.
2
MiR-192-5p-Modified Tumor-Associated Macrophages-Derived Exosome Suppressed Endometrial Cancer Progression Through Targeting IRAK1/NF-κB Signaling.miR-192-5p 修饰的肿瘤相关巨噬细胞衍生的外泌体通过靶向 IRAK1/NF-κB 信号通路抑制子宫内膜癌的进展。
Reprod Sci. 2022 Feb;29(2):436-447. doi: 10.1007/s43032-021-00789-8. Epub 2022 Jan 9.
3
LncRNA TRPM2-AS promotes endometrial carcinoma progression and angiogenesis via targeting miR-497-5p/SPP1 axis.长链非编码 RNA TRPM2-AS 通过靶向 miR-497-5p/SPP1 轴促进子宫内膜癌的进展和血管生成。
Cell Mol Biol Lett. 2024 Jul 2;29(1):93. doi: 10.1186/s11658-024-00612-7.
4
PCOS serum-derived exosomal miR-27a-5p stimulates endometrial cancer cells migration and invasion.多囊卵巢综合征血清来源的外泌体 miR-27a-5p 促进子宫内膜癌细胞的迁移和侵袭。
J Mol Endocrinol. 2020 Jan;64(1):1-12. doi: 10.1530/JME-19-0159.
5
Cancer-associated fibroblasts contribute to oral cancer cells proliferation and metastasis via exosome-mediated paracrine miR-34a-5p.癌相关成纤维细胞通过外泌体介导的旁分泌 miR-34a-5p 促进口腔癌细胞的增殖和转移。
EBioMedicine. 2018 Oct;36:209-220. doi: 10.1016/j.ebiom.2018.09.006. Epub 2018 Sep 20.
6
Exosomal lncRNA NEAT1 from cancer-associated fibroblasts facilitates endometrial cancer progression via miR-26a/b-5p-mediated STAT3/YKL-40 signaling pathway.来自癌症相关成纤维细胞的外泌体 lncRNA NEAT1 通过 miR-26a/b-5p 介导的 STAT3/YKL-40 信号通路促进子宫内膜癌进展。
Neoplasia. 2021 Jul;23(7):692-703. doi: 10.1016/j.neo.2021.05.004. Epub 2021 Jun 18.
7
Transcriptomics of tissue exosomes to investigate miR-195-5p's amelioration of endometrial fibrosis via the YAP-Smad7 pathway: an animal study.组织外泌体的转录组学研究 miR-195-5p 通过 YAP-Smad7 通路改善子宫内膜纤维化：一项动物研究。
J Transl Med. 2024 Nov 21;22(1):1050. doi: 10.1186/s12967-024-05871-8.
8
S-RBD-modified and miR-486-5p-engineered exosomes derived from mesenchymal stem cells suppress ferroptosis and alleviate radiation-induced lung injury and long-term pulmonary fibrosis.S-RBD 修饰和 miR-486-5p 工程化间充质干细胞衍生的外泌体抑制铁死亡，减轻放射性肺损伤和长期肺纤维化。
J Nanobiotechnology. 2024 Oct 26;22(1):662. doi: 10.1186/s12951-024-02830-9.
9
miRNA-576-5p promotes endometrial cancer cell growth and metastasis by targeting ZBTB4.miRNA-576-5p 通过靶向 ZBTB4 促进子宫内膜癌细胞的生长和转移。
Clin Transl Oncol. 2023 Mar;25(3):706-720. doi: 10.1007/s12094-022-02976-8. Epub 2022 Dec 20.
10
Exosomal circRNA RHOT1 promotes breast cancer progression by targeting miR-204-5p/ PRMT5 axis.外泌体环状RNA RHOT1通过靶向miR-204-5p/PRMT5轴促进乳腺癌进展。
Cancer Cell Int. 2023 Nov 3;23(1):260. doi: 10.1186/s12935-023-03111-5.

引用本文的文献

1
Extracellular Vesicle Profiling Reveals Novel Autism Signatures in Patient-Derived Forebrain Organoids.细胞外囊泡分析揭示了患者来源的前脑类器官中的新型自闭症特征。
Res Sq. 2025 May 13:rs.3.rs-6573757. doi: 10.21203/rs.3.rs-6573757/v1.

本文引用的文献

1
The UCSC Genome Browser database: 2025 update.加州大学圣克鲁兹分校基因组浏览器数据库：2025年更新
Nucleic Acids Res. 2025 Jan 6;53(D1):D1243-D1249. doi: 10.1093/nar/gkae974.
2
miR-508-5p serves as an anti-oncogene by targeting S100A16 to regulate AKT signaling and epithelial-mesenchymal transition process in lung adenocarcinoma cells.miR-508-5p 通过靶向 S100A16 调控 AKT 信号通路和上皮-间质转化过程抑制肺腺癌细胞的恶性进展。
Am J Med Sci. 2023 Jun;365(6):520-531. doi: 10.1016/j.amjms.2023.02.014. Epub 2023 Mar 24.
3
Advanced technologies for molecular diagnosis of cancer: State of pre-clinical tumor-derived exosome liquid biopsies.
癌症分子诊断的先进技术：临床前肿瘤来源外泌体液体活检的现状
Mater Today Bio. 2022 Dec 29;18:100538. doi: 10.1016/j.mtbio.2022.100538. eCollection 2023 Feb.
4
Exosome biogenesis: machinery, regulation, and therapeutic implications in cancer.外泌体的生物发生：在癌症中的机制、调控和治疗意义。
Mol Cancer. 2022 Nov 1;21(1):207. doi: 10.1186/s12943-022-01671-0.
5
miRNA: A Promising Therapeutic Target in Cancer.miRNA：癌症治疗的新靶点。
Int J Mol Sci. 2022 Sep 29;23(19):11502. doi: 10.3390/ijms231911502.
6
A review on exosomes application in clinical trials: perspective, questions, and challenges.外泌体在临床试验中的应用综述：观点、问题与挑战。
Cell Commun Signal. 2022 Sep 19;20(1):145. doi: 10.1186/s12964-022-00959-4.
7
Mesenchymal stem cell-derived exosomes in cancer therapy resistance: recent advances and therapeutic potential.间质干细胞衍生的外泌体在癌症治疗耐药中的作用：最新进展和治疗潜力。
Mol Cancer. 2022 Sep 13;21(1):179. doi: 10.1186/s12943-022-01650-5.
8
Endometrial cancer.子宫内膜癌。
Lancet. 2022 Apr 9;399(10333):1412-1428. doi: 10.1016/S0140-6736(22)00323-3.
9
Drug Discovery of DKK1 Inhibitors.DKK1抑制剂的药物研发
Front Pharmacol. 2022 Mar 9;13:847387. doi: 10.3389/fphar.2022.847387. eCollection 2022.
10
UPF1 contributes to the maintenance of endometrial cancer stem cell phenotype by stabilizing LINC00963.UPF1 通过稳定 LINC00963 促进子宫内膜癌干细胞表型的维持。
Cell Death Dis. 2022 Mar 22;13(3):257. doi: 10.1038/s41419-022-04707-x.