Department of Genetic and Bioengineering, Faculty of Engineering and Natural Sciences , Gumushane University , Gumushane , Turkiye.
Department of Nutrition and Dietetics, Faculty of Health Sciences , Karadeniz Technical University , Trabzon , Turkiye.
Nutr Cancer. 2019;71(7):1181-1188. doi: 10.1080/01635581.2019.1598563. Epub 2019 Apr 8.
Although several studies have investigated the cytotoxic effects of different species, there has been only limited research into the cytotoxic effect of The purpose of this research was to evaluate the phenolic characterization and the cytotoxic effect of on human colon cancer (WiDr) cells and the possible mechanisms involved. Total polyphenolic contents (TPC) and phenolic characterization of the extract were evaluated using the Folin-Cioceltau method and reversed-phase high performance liquid chromatography (RP-HPLC), respectively. The cytotoxic activity of the extract was determined using the methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay. The mechanism involved in the extract's cytotoxic effect was then evaluated in terms of apoptosis and the cell cycle using flow cytometry, while mitochondrial membrane potential (MMP) was investigated using the fluorometric method. The TPC value of the extract was 784.8 ± 40.3 mg gallic acid equivalent per 100 g sample, and sinapic acid and benzoic acid were detected as major phenolics in the extract. extract exhibited a selective cytotoxic effect (3.6-fold) on WiDr cells compared to normal colon cells. The extract induced cell cycle arrest at the S phase and apoptosis via reduced MMP in WiDr cells. Phytomedical and nutraceutical applications of may represent promising approaches in the treatment of cancer.
虽然已有多项研究调查了不同物种的细胞毒性作用，但对的细胞毒性作用的研究却很有限。本研究旨在评估对人结肠癌细胞（WiDr）的酚类特性和细胞毒性作用，以及可能涉及的机制。采用福林-考尔特法和反相高效液相色谱法（RP-HPLC）分别评估总多酚含量（TPC）和提取物的酚类特性。采用甲基噻唑基二苯基四唑溴盐（MTT）法测定提取物的细胞毒性活性。然后，通过流式细胞术评估提取物的细胞毒性作用涉及的凋亡和细胞周期，同时通过荧光法研究线粒体膜电位（MMP）。提取物的 TPC 值为 784.8±40.3mg 没食子酸当量/100g 样品，提取物中检测到的主要酚类为芥子酸和苯甲酸。与正常结肠细胞相比，提取物对 WiDr 细胞表现出选择性细胞毒性作用（3.6 倍）。提取物通过降低 WiDr 细胞中的 MMP 诱导细胞周期停滞在 S 期和凋亡。的植物药和营养保健品应用可能代表癌症治疗的有前途的方法。
