Department of Nutrition and Dietetics, Faculty of Health Sciences, Karadeniz Technical University, Trabzon, Turkiye.
Department of Medical Biochemistry, Faculty of Medicine, Karadeniz Technical University, Trabzon, Turkiye.
Nutr Cancer. 2020;72(3):504-512. doi: 10.1080/01635581.2019.1636100. Epub 2019 Jul 10.
Although several studies have investigated the cytotoxic effects of different species, limited researches have been conducted on the cytotoxic effect of . The aim of this study was to evaluate the phenolic characterization and the cytotoxic effect of on human cervix (HeLa) and colon (WiDr) cancer cells and the possible mechanisms involved. Total phenolic content (TPC) and phenolic characterization of the extract were investigated using the Folin-Cioceltau method and RP-HPLC, respectively. The cytotoxic effect of the extract was evaluated using the MTT assay. The mechanism involved in the extract's cytotoxic effect was then evaluated in terms of apoptosis and the cell cycle using flow cytometry, while mitochondrial membrane potential (MMP) was investigated using the fluorometric method. The TPC value of the extract was 141.2 ± 0.8 mg gallic acid equivalent per g sample, and quercetin was detected as major phenolics. extract exhibited a selective cytotoxic effect on HeLa and WiDr cells compared to normal fibroblast and colon cells, respectively. The extract induced cell cycle arrest at the S phase and apoptosis via reduced MMP in these cells. Further studies may be useful in developing a natural product based new generation pharmacological agent.
虽然已有多项研究调查了不同物种的细胞毒性作用，但对 的细胞毒性作用的研究却很有限。本研究旨在评估 的酚类特性及其对人宫颈（HeLa）和结肠（WiDr）癌细胞的细胞毒性作用，以及涉及的可能机制。采用 Folin-Ciocalteu 法和 RP-HPLC 分别研究了提取物的总酚含量（TPC）和酚类特性。采用 MTT 法评估提取物的细胞毒性作用。然后，通过流式细胞术评估提取物细胞毒性作用涉及的凋亡和细胞周期，同时采用荧光法研究线粒体膜电位（MMP）。提取物的 TPC 值为 141.2 ± 0.8 mg 没食子酸当量/克样品，检测到槲皮素为主要酚类。与正常成纤维细胞和结肠细胞相比， 提取物对 HeLa 和 WiDr 细胞具有选择性细胞毒性作用。该提取物通过降低这些细胞中的 MMP，诱导细胞周期停滞在 S 期并诱导细胞凋亡。进一步的研究可能有助于开发基于天然产物的新一代药理学制剂。
