Owumi Solomon E, Danso Olabisi F, Effiong Magdalene E
Cancer Research and Molecular Biology Laboratories, Department of Biochemistry, College of Medicine, University of Ibadan, Ibadan, Nigeria.
Industrial and Nutritional Biochemistry, Department of Biochemistry, College of Medicine, University of Ibadan, Ibadan, Nigeria.
Acta Biochim Pol. 2019 Apr 8;66(2):201-206. doi: 10.18388/abp.2018_2771.
Exposure to dichloromethane (DCM), a commonly used chlorinated solvent in industrial settings and for the production of many household products, reportedly elicits detrimental effects in animals and humans. The present study investigated the protective role of dietary quercetin on DCM-induced hepatorenal damage in rats. Experimental rats were orally administered with DCM (150 mg/kg) and 30 min later with quercetin at 10, 20 and 40 mg/kg or none for 7 consecutive days. The results indicated that DCM-mediated significant (p<0.05) increases in serum alanine aminotransferase, aspartate aminotransferase, gamma glutamyl transferase and alkaline phosphatase activities as well as urea and creatinine levels were dose-dependently normalized to the control values in rats co-treated with quercetin. Further, quercetin co-treatment ameliorated DCM-mediated decrease in the hepatic and renal activities of superoxide dismutase, catalase, glutathione peroxidase and glutathione S-transferase as well as glutathione level in the treated rats. Moreover, quercetin co-treatment markedly reduced lipid peroxidation level and protected against histological changes in liver and kidney of the treated rats. Taken together, quercetin abrogated hepatorenal oxidative damage in DCM-treated rats via improvement of antioxidant status and suppression of oxidative damage.
二氯甲烷（DCM）是工业环境中常用的氯化溶剂，也是许多家用产品生产过程中常用的溶剂。据报道，DCM会对动物和人类产生有害影响。本研究调查了膳食槲皮素对DCM诱导的大鼠肝肾损伤的保护作用。实验大鼠口服给予DCM（150 mg/kg），30分钟后分别给予10、20和40 mg/kg的槲皮素或不给药，连续7天。结果表明，DCM导致血清丙氨酸氨基转移酶、天冬氨酸氨基转移酶、γ-谷氨酰转移酶和碱性磷酸酶活性以及尿素和肌酐水平显著（p<0.05）升高，而在与槲皮素共同处理的大鼠中，这些指标呈剂量依赖性恢复至对照值。此外，槲皮素共同处理改善了DCM介导的处理大鼠肝脏和肾脏中超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶和谷胱甘肽S-转移酶活性以及谷胱甘肽水平的降低。此外，槲皮素共同处理显著降低了脂质过氧化水平，并防止了处理大鼠肝脏和肾脏的组织学变化。综上所述，槲皮素通过改善抗氧化状态和抑制氧化损伤，消除了DCM处理大鼠的肝肾氧化损伤。
