Jung Jin-Song, Ko Su-Joung, Lee Hong-Beom, Lee Su-Bin, Kim Hyoung-Jun, Oh Jae-Min
Department of Chemistry and Medical Chemistry, College of Science and Technology, Yonsei University, Wonju, Gangwondo 26493, Korea.
Polymers (Basel). 2019 Jan 17;11(1):155. doi: 10.3390/polym11010155.
A hierarchical silver nanostructure with improved antibacterial property was fabricated utilizing silver coordination polymer. Octadecanethiolate⁻silver polymer was synthesized to have a layered structure and was coated on silicon wafer by drop-casting method utilizing hydrophobic⁻hydrophobic interaction. Thus, the silver coordination polymer was calcined under reductive condition to produce zero-valent silver with a hierarchical nanostructure. X-ray diffraction patterns revealed that layered silver coordination polymer successfully transformed to hexagonal silver upon calcination. According to scanning electron and atomic force microscopy, silver coordination polymer with ~145.5 nm size was homogeneously coated on the surface before calcination, and it evolved micrometer-sized lumps and grooves which were composed of ~58.8 nm sized Ag nanoparticles. The hierarchical structure-micrometer lump/groove consisting of Ag nanoparticles-would be advantageous to kill bacteria; micrometer-grooves provide physical condition (pocket for bacteria capture) and the Ag nanoparticles from the neighboring lump endow chemical condition (antibacterial property of released Ag⁺). The antibacterial activity test on via colony forming inhibitory assay indeed exhibited an improved antibacterial activity of hierarchical Ag nanostructure compared with the surface simply coated with Ag nanoparticles. From the line profile of atomic force microscopy, the bacterium trapped in the hierarchical Ag nanostructure was shown to interact intimately with Ag surface.
利用银配位聚合物制备了具有增强抗菌性能的分级银纳米结构。合成了十八烷硫醇银聚合物以形成层状结构，并利用疏水-疏水相互作用通过滴铸法将其涂覆在硅片上。因此，在还原条件下煅烧银配位聚合物以产生具有分级纳米结构的零价银。X射线衍射图谱表明，层状银配位聚合物在煅烧后成功转变为六方银。根据扫描电子显微镜和原子力显微镜观察，煅烧前尺寸约为145.5 nm的银配位聚合物均匀地涂覆在表面上，煅烧后演变成由尺寸约为58.8 nm的银纳米颗粒组成的微米级块状物和沟槽。由银纳米颗粒组成的分级结构——微米级块状物/沟槽——将有利于杀灭细菌；微米级沟槽提供物理条件（捕获细菌的口袋），相邻块状物中的银纳米颗粒赋予化学条件（释放的Ag⁺的抗菌性能）。通过菌落形成抑制试验对……进行的抗菌活性测试确实表明，与仅涂覆有银纳米颗粒的表面相比，分级银纳米结构具有增强的抗菌活性。从原子力显微镜的线轮廓图可以看出，被困在分级银纳米结构中的细菌与银表面有密切的相互作用。
