Lang Yanhe, Sun Yuan, Yu Miao, Ji Yubin, Wang Lei, Zhang Zhizhou
Key Laboratory of Saline-alkali Vegetation Ecology Restoration in Oil Field (SAVER), Ministry of Education, Alkali Soil Natural Environmental Science Center (ASNESC), Northeast Forestry University, Harbin 150040, China.
Center of Marine Antifouling Engineering Technology of Shandong Province, School of Marine Science and Technology, Harbin Institute of Technology at Weihai, Weihai 264209, China.
Polymers (Basel). 2019 Jan 17;11(1):161. doi: 10.3390/polym11010161.
In this study, the actual anti-biofouling (AF) efficacy of three protective coatings, including a chlorinated rubber-based coating (C₀) and two polydimethylsiloxane (PDMS)-based coatings (P₀ and P), were estimated via the static field exposure assays. The surface properties of these protective coatings, including surface wettability and morphology features, were characterized using the static water contact angle (WCA) and scanning electron microscope (SEM). The colonization and succession dynamics of the early-adherent biofilm-forming eukaryotic microbial communities occupied on these protective coatings were explored using the Single-stranded Conformation Polymorphism (SSCP) technique. The field data clearly revealed that coating P₀ and P performed better in the long-term static submergence, as compared with the C₀ surface, while coating P showed excellent AF efficacy in the field. Fingerprinting analysis suggested that the diversity, abundance, the clustering patterns, and colonization dynamics of the early-colonized eukaryotic microbes were significantly perturbed by these protective coatings, particularly by the P surfaces. These differential AF efficacy and perturbation effects would be largely ascribed to the differences in the wettability and surface nanostructures between the C₀, P₀ and P surfaces, as evidenced by WCA and SEM analysis.
在本研究中，通过静态现场暴露试验评估了三种防护涂层的实际抗生物污损（AF）效果，这三种涂层包括一种氯化橡胶基涂层（C₀）和两种聚二甲基硅氧烷（PDMS）基涂层（P₀和P）。使用静态水接触角（WCA）和扫描电子显微镜（SEM）对这些防护涂层的表面性质，包括表面润湿性和形态特征进行了表征。使用单链构象多态性（SSCP）技术探索了占据在这些防护涂层上的早期附着生物膜形成真核微生物群落的定殖和演替动态。现场数据清楚地表明，与C₀表面相比，涂层P₀和P在长期静态浸没中表现更好，而涂层P在现场显示出优异的AF效果。指纹分析表明，这些防护涂层，特别是P表面，显著扰乱了早期定殖真核微生物的多样性、丰度、聚类模式和定殖动态。如WCA和SEM分析所示，这些不同的AF效果和扰动效应在很大程度上归因于C₀、P₀和P表面之间润湿性和表面纳米结构的差异。
