Yang Jin-Long, Li Yi-Feng, Guo Xing-Pan, Liang Xiao, Xu Yue-Feng, Ding De-Wen, Bao Wei-Yang, Dobretsov Sergey
a Marine Ecology Research Center , The First Institute of Oceanography, State Oceanic Administration , Qingdao , PR China.
b Department of Biology, Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources , Shanghai Ocean University, Ministry of Education , Shanghai , PR China.
Biofouling. 2016 Aug;32(7):763-77. doi: 10.1080/08927014.2016.1197210.
This study investigated the effect of carbon nanotubes (CNTs) and titanium dioxide (TiO2) incorporated in PDMS on biofilm formation and plantigrade settlement of Mytilus coruscus. TiO2 increased bacterial density, and CNTs also increased bacterial density but reduced diatom density in biofilms after 28 days. Further analysis was conducted between bacterial communities on glass, PDMS, CNTs (0.5 wt%) and TiO2 (7.5 wt%). ANOSIM analysis revealed significant differences (R > 0.9) between seven, 14, 21 and 28 day-old bacterial communities. MiSeq sequencing showed that CNTs and TiO2 impacted the composition of 28 day-old bacterial communities by increasing the abundance of Proteobacteria and decreasing the abundance of Bacteroidetes. The maximum decreased settlement rate in 28 day-old biofilms on CNTs and TiO2 was > 50% in comparison to those on glass and PDMS. Thus, CNTs and TiO2 incorporated in PDMS altered the biomass and community composition of biofilms, and subsequently decreased mussel settlement.
本研究调查了聚二甲基硅氧烷（PDMS）中掺入的碳纳米管（CNTs）和二氧化钛（TiO₂）对波纹巴非蛤生物膜形成和爬行定居的影响。28天后，TiO₂增加了生物膜中的细菌密度，CNTs也增加了细菌密度，但降低了硅藻密度。对玻璃、PDMS、CNTs（0.5 wt%）和TiO₂（7.5 wt%）表面的细菌群落进行了进一步分析。ANOSIM分析显示，7天、14天、21天和28天龄的细菌群落之间存在显著差异（R > 0.9）。MiSeq测序表明，CNTs和TiO₂通过增加变形菌门的丰度和降低拟杆菌门的丰度，影响了28天龄细菌群落的组成。与玻璃和PDMS表面相比，28天龄生物膜在CNTs和TiO₂表面的最大定居率下降>50%。因此，PDMS中掺入的CNTs和TiO₂改变了生物膜的生物量和群落组成，进而降低了贻贝的定居率。
