Willig R P, Braun W, Commentz J C, Stahnke N
Acta Endocrinol Suppl (Copenh). 1986;279:411-5. doi: 10.1530/acta.0.112s411.
In 3 groups of 8 children and adolescents each with Prader-Willi-Labhart's Syndrome (PW-S), obese patients matched for body weight (control I), and normal weight subjects matched for pubertal stage (control II) plasma concentrations of melatonin, cortisol, growth hormone (hGH), insulin, gonadal hormones, and gonadotropins were measured every 1 to 4 hours in 24-hour-profiles. All hormones were determined by radioimmunoassay. The specific melatonin antibody was raised in rabbits. Criteria of the melatonin assay were as follows: detection limit for plasma concentrations of 13 pg/ml, intraassay and interassay variations: 8.4 and 11.2%, respectively. PW-S-patients showed cortisol fluctuations within normal limits. hGH was lower than 5 micrograms/l even during sleep, insulin ranged between 5 and 170 mU/l, and no excessively high glucose levels were found. Estradiol and testosterone were low for age and for pubertal development in all patients except in two girls. Basal LH and FSH levels were in the low normal range and showed sluggish response to LHRH. Plasma melatonin was low during the day, increased at mid-night and peaked at 3 a.m. Melatonin levels in PW-S were not significantly different from those in both control groups. We concluded that the impairment of gonadotropin secretion in patients with PW-S is not due to elevated levels of plasma melatonin.
在3组研究对象中，每组各有8名患有普拉德-威利-拉布哈特综合征（PW-S）的儿童和青少年、体重匹配的肥胖患者（对照组I）以及青春期阶段匹配的正常体重受试者（对照组II），通过24小时监测，每1至4小时测量一次褪黑素、皮质醇、生长激素（hGH）、胰岛素、性腺激素和促性腺激素的血浆浓度。所有激素均采用放射免疫分析法测定。特异性褪黑素抗体是在兔体内产生的。褪黑素测定的标准如下：血浆浓度检测限为13 pg/ml，批内和批间变异系数分别为8.4%和11.2%。PW-S患者的皮质醇波动在正常范围内。即使在睡眠期间，hGH也低于5微克/升，胰岛素水平在5至170 mU/升之间，未发现血糖水平过高。除两名女孩外，所有患者的雌二醇和睾酮水平均低于其年龄和青春期发育水平。基础促黄体生成素（LH）和促卵泡生成素（FSH）水平处于正常低限，对促黄体生成素释放激素（LHRH）反应迟缓。血浆褪黑素在白天较低，午夜时升高，并在凌晨3点达到峰值。PW-S患者的褪黑素水平与两个对照组相比无显著差异。我们得出结论，PW-S患者促性腺激素分泌受损并非由于血浆褪黑素水平升高所致。
