Tang Xiaogang, Ding Qiulong, Chen Chao, Chen Fei, Zhou Xiyang, Hong Chu Jie, Pan Wensheng
Pharmazie. 2019 Mar 1;74(3):175-178. doi: 10.1691/ph.2019.8816.
Upregulation of pro-inflammatory cytokine interleukin (IL)-6 is observed in gastric cancer tissue, and high IL-6 serum levels predict a poor prognosis of gastric cancer patients. The IL-6/STAT3 pathway has been confirmed to play essential roles in the process of carcinogenesis, including gastric cancer. Thus, blockade of the IL-6/STAT3 pathway may be a potentially effective therapeutic option for gastric cancer. Micheliolide (MCL), a guaianolide sesquiterpene lactone, possesses anti-inflamma tory properties and can attenuate the IL-6 level. In addition, MCL has been widely reported to possess anti-tumor activity. But the anti-cancer effect of MCL on gastric cancer is unclear. In this study, we detected the effects of MCL on gastric cancer cell proliferation and apoptosis by performing MTT, colony formation, TUNEL and western blot assays, and found that MCL inhibited gastric cancer cell proliferation and promoted apoptosis We further investigated the molecular mechanism by which MCL played an efficient role against gastric cancer, and found that the IL-6/STAT3 pathway is involved in the anti-cancer effect of MCL on gastric cancer. experiments further confirmed this conclusion. Taken together, MCL inhibits gastric cancer growth and via blockade of IL-6/STAT3 pathway.
在胃癌组织中观察到促炎细胞因子白细胞介素（IL）-6的上调，且血清IL-6水平升高预示着胃癌患者预后不良。IL-6/信号转导和转录激活因子3（STAT3）通路已被证实在包括胃癌在内的致癌过程中发挥重要作用。因此，阻断IL-6/STAT3通路可能是胃癌一种潜在有效的治疗选择。米契罗内酯（MCL）是一种愈创木烷型倍半萜内酯，具有抗炎特性，可降低IL-6水平。此外，已有广泛报道称MCL具有抗肿瘤活性。但MCL对胃癌的抗癌作用尚不清楚。在本研究中，我们通过MTT、集落形成、TUNEL和蛋白质印迹分析检测了MCL对胃癌细胞增殖和凋亡的影响，发现MCL抑制胃癌细胞增殖并促进凋亡。我们进一步研究了MCL对胃癌发挥有效作用的分子机制，发现IL-6/STAT3通路参与了MCL对胃癌的抗癌作用。实验进一步证实了这一结论。综上所述，MCL通过阻断IL-6/STAT3通路抑制胃癌生长。
