Meador V P, Tabatabai L B, Hagemoser W A, Deyoe B L
Am J Vet Res. 1986 Oct;47(10):2147-50.
An avidin-biotin-peroxidase complex immunoenzymatic staining technique was evaluated for light microscopic detection of Brucella organisms in formalin-fixed, paraffin-embedded tissues. Tissues from cows, goats, and mice inoculated with B abortus strain 2308 were examined, using rabbit antiserum to Brucella cell surface protein as primary antibody. Stained organisms were identified histologically in tissue sections containing B abortus, as detected by bacteriologic examination of duplicate nonprocessed tissue samples. Bacillus sp, Corynebacterium pyogenes, Escherichia coli, Listeria monocytogenes, Pasteurella haemolytica, P multocida, Salmonella typhimurium, Staphylococcus sp, and Streptococcus sp did not stain with this system, using anti-Brucella cell surface protein primary antibody, thus indicating specificity for Brucella organisms.
对抗生物素蛋白-生物素-过氧化物酶复合物免疫酶染色技术进行了评估，以用于在福尔马林固定、石蜡包埋组织中对布鲁氏菌进行光学显微镜检测。使用兔抗布鲁氏菌细胞表面蛋白血清作为一抗，检查了接种流产布鲁氏菌2308菌株的牛、山羊和小鼠的组织。通过对重复的未处理组织样本进行细菌学检查检测到，在含有流产布鲁氏菌的组织切片中，经组织学鉴定出染色的微生物。使用抗布鲁氏菌细胞表面蛋白一抗时，芽孢杆菌属、化脓棒状杆菌、大肠杆菌、单核细胞增生李斯特菌、溶血巴斯德菌、多杀巴斯德菌、鼠伤寒沙门氏菌、葡萄球菌属和链球菌属在该系统中未染色，因此表明该技术对布鲁氏菌具有特异性。
