Stevens M G, Olsen S C, Pugh G W, Palmer M V
Brucellosis Research Unit, National Animal Disease Center, USDA Agricultural Research Service, Ames, Iowa 50010.
Infect Immun. 1994 Aug;62(8):3206-12. doi: 10.1128/iai.62.8.3206-3212.1994.
Immune and pathologic responses were measured for 20 weeks after infection of mice with Brucella abortus 19, RB51, or 2308. Live bacteria and bacterial antigens of 19 and RB51 persisted in spleens for 10 and 4 weeks after infection, respectively, whereas 2308 bacteria and bacterial antigens persisted for at least 20 weeks. Small germinal centers and profound lymphoid depletion occurred in spleens of mice during the first 4 weeks of infection with strain 19 or 2308; however, mice infected with strain RB51 had much larger germinal centers but no lymphoid depletion. At 4 weeks, only spleen cells from RB51-infected mice proliferated when incubated with 2308 bacteria. Large germinal centers in the spleen and spleen cell proliferative responses to 2308 did not appear in strain 19-infected mice until 6 weeks or in strain 2308-infected mice until 10 weeks. Similar proliferative responses to 2308 occurred in mice infected with strain 19 or RB51 at 6 weeks and in mice infected with strain 19, RB51, or 2308 at 10 weeks. However, at 20 weeks, spleen cell proliferative responses to 2308 occurred in mice infected with strain 19 or 2308 but not in mice infected with strain RB51. Mice infected with strain RB51 had lower and less persistent antibody titers to 2308 than did mice infected with strain 19 or 2308. Collectively, these results indicate that RB51-infected mice have less persistent immune responses to 2308 than do mice infected with 19 or 2308. The shorter duration of the responses probably resulted because RB51 is considerably less pathogenic and is cleared more rapidly from mice than are 19 and 2308.
在用流产布鲁氏菌19、RB51或2308感染小鼠后，对其免疫和病理反应进行了20周的监测。19型和RB51型的活菌及细菌抗原分别在感染后10周和4周持续存在于脾脏中，而2308型细菌及细菌抗原持续存在至少20周。在用19型或2308型菌株感染小鼠的前4周，脾脏中出现小生发中心和严重的淋巴细胞耗竭；然而，感染RB51型菌株的小鼠生发中心大得多，但没有淋巴细胞耗竭。在4周时，仅RB51感染小鼠的脾细胞与2308型细菌一起孵育时会增殖。直到6周，19型感染小鼠的脾脏中才出现大生发中心以及脾细胞对2308型的增殖反应，而2308型感染小鼠直到10周才出现。19型或RB51型感染小鼠在6周时以及19型、RB51型或2308型感染小鼠在10周时，对2308型有类似的增殖反应。然而，在20周时，19型或2308型感染小鼠的脾细胞对2308型有增殖反应，而RB51型感染小鼠则没有。与感染19型或2308型的小鼠相比，感染RB51型的小鼠对2308型的抗体滴度更低且持续时间更短。总体而言，这些结果表明，与感染19型或2308型的小鼠相比，感染RB51型的小鼠对2308型的免疫反应持续时间更短。反应持续时间较短可能是因为RB51的致病性相当低，并且比19型和2308型从小鼠体内清除得更快。
