自然感染家鸭（绿头鸭家鸭品种）的禽结核继发的淀粉样蛋白A淀粉样变性。

Amyloid A amyloidosis secondary to avian tuberculosis in naturally infected domestic pekin ducks (Anas platyrhynchos domestica).

作者信息

Chen Hongxi, Zhu Dekang, Wang Mingshu, Jia Renyong, Chen Shun, Liu Mafeng, Zhao Xinxin, Yang Qiao, Wu Ying, Zhang Shaqiu, Liu Yunya, Zhang Ling, Yu Yanling, Chen Xiaoyue, Cheng Anchun

机构信息

Research Center of Avian Diseases, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu, Sichuan, China.

出版信息

Comp Immunol Microbiol Infect Dis. 2019 Apr;63:136-141. doi: 10.1016/j.cimid.2019.01.016. Epub 2019 Feb 2.

DOI:10.1016/j.cimid.2019.01.016

PMID:30961809

Abstract

To investigate the correlation between avian tuberculosis and duck amyloidosis, the liver, lung, spleen, kidney, duodenum and pectoralis muscle of ducks naturally infected with Mycobacterium avium subsp. avium were used to detect amyloidosis by Congo red staining and potassium permanganate-Congo red staining. The expression level of IL-1β, IL-6, IL-10, TNF-α and SAA2 were detected by quantitative real-time RT-PCR (qRT-PCR). The results showed that the liver, lung, spleen, kidney, duodenum and pectoralis muscle of the infected ducks exhibited amyloid proteins under ordinary light microscopy and the polarization light under polarized light microscopy. However, no amyloid deposition in potassium permanganate-Congo red staining sections indicated that the amyloidosis was AA amyloidosis. In addition, the expression level of IL-1β, IL-6, IL-10, TNF-α and SAA2 increased from 4 to 43. This study showed that avian tuberculosis could induce secondary amyloidosis in naturally infected ducks.

摘要

为研究禽结核病与鸭淀粉样变之间的相关性，采用刚果红染色和高锰酸钾-刚果红染色法，对自然感染鸟分枝杆菌鸟亚种的鸭的肝脏、肺、脾脏、肾脏、十二指肠和胸肌进行淀粉样变检测。通过定量实时逆转录聚合酶链反应（qRT-PCR）检测白细胞介素-1β（IL-1β）、白细胞介素-6（IL-6）、白细胞介素-10（IL-10）、肿瘤坏死因子-α（TNF-α）和血清淀粉样蛋白A2（SAA2）的表达水平。结果显示，在普通光学显微镜和偏振光显微镜下，感染鸭的肝脏、肺、脾脏、肾脏、十二指肠和胸肌均呈现淀粉样蛋白。然而，高锰酸钾-刚果红染色切片中未出现淀粉样沉积，表明该淀粉样变为AA型淀粉样变。此外，IL-1β、IL-6、IL-10、TNF-α和SAA2的表达水平从4倍增加到43倍。本研究表明，禽结核病可在自然感染的鸭中诱发继发性淀粉样变。