Schulze C, Brügmann M, Böer M, Brandt H P, Pohlenz J, Linke R P
Institut für Pathologie, Tierärztliche Hochschule Hannover, Germany.
Vet Pathol. 1998 Jan;35(1):70-4. doi: 10.1177/030098589803500108.
Generalized amyloidosis with predominant renal medullary amyloid deposition was found in four closely related Siberian tigers (Panthera tigris altaica) suffering from end stage kidney diseases. Only minimal to mild amounts of amyloid were deposited in various organs outside the kidneys with individually variable organ involvement. The Congo red staining affinity of amyloid deposits was sensitive to potassium permanganate oxidation. The deposits were further characterized as being of the amyloid-A (AA) type by immunohistochemistry using the mouse monoclonal antibody mc4 directed against a conserved region of the human AA-protein. A combination of immunohistochemistry and Congo red staining was much more sensitive for the diagnosis of amyloid deposits than Congo red staining alone. With this combination, even minimal amyloid deposits were detected that had been missed in the first reading using Congo-red-stained slides alone. Since no common primary cause was identified, the amyloidosis was classified as idiopathic generalized AA-amyloidosis with a potential familial predisposition.
在四只患有终末期肾病的密切相关的东北虎（Panthera tigris altaica）中发现了以肾髓质淀粉样蛋白沉积为主的全身性淀粉样变性。在肾脏以外的各种器官中，仅沉积了少量至中等量的淀粉样蛋白，各器官受累情况各不相同。淀粉样蛋白沉积物的刚果红染色亲和力对高锰酸钾氧化敏感。通过使用针对人AA蛋白保守区域的小鼠单克隆抗体mc4进行免疫组织化学，沉积物进一步被鉴定为淀粉样蛋白A（AA）型。免疫组织化学和刚果红染色相结合对淀粉样蛋白沉积物的诊断比单独使用刚果红染色更为敏感。通过这种组合，甚至检测到了仅使用刚果红染色玻片初读时遗漏的微量淀粉样蛋白沉积物。由于未发现共同的原发性病因，淀粉样变性被归类为具有潜在家族易感性的特发性全身性AA淀粉样变性。
