Luo Chunying, Pu Jian, Liu Fahui, Long Xidai, Wang Chunfang, Wei Huamei, Tang Qianli
Department of Pathology, Affiliated Hospital of Youjiang Medical College for Nationalities, Guangxi, 533000, PR China; Clinical Medical Research Center of Hepatobiliary Diseases, Affiliated Hospital of Youjiang Medical College for Nationalities, Guangxi, 533000, PR China.
Clinical Medical Research Center of Hepatobiliary Diseases, Affiliated Hospital of Youjiang Medical College for Nationalities, Guangxi, 533000, PR China.
Clin Res Hepatol Gastroenterol. 2019 Nov;43(6):715-721. doi: 10.1016/j.clinre.2019.03.005. Epub 2019 Apr 6.
Accumulating evidences have shown that microRNA-200c (miR-200c) expression is associated with the prognosis of many types of human cancer. However, the prognostic value of miR-200c in hepatocellular carcinoma (HCC) is still unknown. In the present study, the expression of miR-200c in paired HCC and adjacent non-tumor tissues from 148 patients was determined by quantitative real-time PCR (qRT-PCR), and we analyzed its association with clinicopathological characteristics and prognosis of HCC patients. Our results showed that the expression of miR-200c was significantly decreased in HCC tissues compared with adjacent non-tumor tissues (P < 0.0001). Correlation analysis showed that miR-200c expression was significantly associated with tumor size (P = 0.021), serum AFP level (P = 0.016), TNM stage (P = 0.019) and vein invasion (P = 0.026). Patients with lower miR-200c expression had significantly worse overall survival (OS, P = 0.023) and recurrence-free survival (RFS, P = 0.002). The multivariate Cox regression analysis revealed that miR-200c expression was an independent prognostic factor for OS (hazard ratio (HR) [95% CI] = 2.226 [1.235-4.012], P = 0.008) and RFS (HR [95% CI] = 2.662 [1.618-4.38], P < 0.001). In conclusion, our results suggest that the miR-200c expression was significantly down-regulated and associated with poor prognosis in HCC.
越来越多的证据表明，微小RNA-200c（miR-200c）的表达与多种人类癌症的预后相关。然而，miR-200c在肝细胞癌（HCC）中的预后价值仍不清楚。在本研究中，通过定量实时PCR（qRT-PCR）检测了148例患者配对的HCC组织和癌旁非肿瘤组织中miR-200c的表达，并分析了其与HCC患者临床病理特征及预后的关系。我们的结果显示，与癌旁非肿瘤组织相比，miR-200c在HCC组织中的表达显著降低（P＜0.0001）。相关性分析表明，miR-200c表达与肿瘤大小（P = 0.021）、血清甲胎蛋白水平（P = 0.016）、TNM分期（P = 0.019）和静脉侵犯（P = 0.026）显著相关。miR-200c表达较低的患者总生存期（OS，P = 0.023）和无复发生存期（RFS，P = 0.002）明显较差。多因素Cox回归分析显示，miR-200c表达是OS（风险比（HR）[95%可信区间] = 2.226 [1.235 - 4.012]，P = 0.008）和RFS（HR [95%可信区间] = 2.662 [1.618 - 4.38]，P＜0.001）的独立预后因素。总之，我们的结果表明，miR-200c表达在HCC中显著下调并与不良预后相关。
