State Key Laboratory of Oral Diseases and National Clinical Research Center for Oral Diseases, Department of Oral and Maxillofacial Surgery, West China Hospital of Stomatology, Sichuan University, Chengdu, 610000, China.
Department of Stomatology, The First Affiliated Hospital of Dalian Medical University, Dalian, 116000, China.
Int J Clin Oncol. 2020 Jun;25(6):1072-1078. doi: 10.1007/s10147-020-01649-2. Epub 2020 Mar 11.
MicroRNAs (miRNAs) are considered as promising cancer biomarkers. The aim of the present study is to investigate the prognostic significance of miR-200c in patients with oral squamous cell carcinoma (OSCC).
Quantitative real-time PCR (qRT-PCR) was used to determine the expression levels of miR-200c in 204 pairs of OSCC and adjacent noncancerous. Correlations between miR-200c expression levels and clinicopathological characteristics were investigated. Survival analysis was performed using the Kaplan-Meier method and log-rank test. Multivariate analysis of the prognostic factors was performed with a Cox proportional hazards regression model.
The expression of miR-200c was significantly down-regulated in OSCC tissues compared with adjacent non-tumor tissues (p < 0.0001). Low expression of miR-200c in tumor tissues was significantly correlated with the positive N classification (p = 0.013), advanced TNM stage (p = 0.007) and poor differentiation grade (p = 0.026). Lower miR-200c expression in patients was significantly associated with poor recurrence-free survival (RFS, p = 0.0003) and overall survival (OS, p = 0.0026). Multivariate analysis confirmed that low miR-200c expression was an independent predictor for poor RFS (hazard ratio (HR) 1.705, 95% CI 1.136-2.56, p = 0.01) and OS (HR 1.669, 95% CI 1.03-2.703, p = 0.037) in patients with OSCC.
Our results suggest that the miR-200c might be a potential prognostic biomarker for OSCC.
微小 RNA（miRNAs）被认为是有前途的癌症生物标志物。本研究旨在探讨 miR-200c 在口腔鳞状细胞癌（OSCC）患者中的预后意义。
采用实时定量 PCR（qRT-PCR）检测 204 对 OSCC 及相邻非癌组织中 miR-200c 的表达水平。分析 miR-200c 表达水平与临床病理特征的相关性。采用 Kaplan-Meier 法和对数秩检验进行生存分析。采用 Cox 比例风险回归模型进行预后因素的多因素分析。
miR-200c 在 OSCC 组织中的表达明显低于相邻非肿瘤组织（p<0.0001）。肿瘤组织中 miR-200c 的低表达与 N 分类阳性（p=0.013）、TNM 分期较晚（p=0.007）和分化程度差（p=0.026）显著相关。患者 miR-200c 表达水平较低与无复发生存率（RFS，p=0.0003）和总生存率（OS，p=0.0026）显著相关。多因素分析证实，miR-200c 低表达是 OSCC 患者 RFS（风险比（HR）1.705，95%CI 1.136-2.56，p=0.01）和 OS（HR 1.669，95%CI 1.03-2.703，p=0.037）的独立预测因子。
我们的研究结果表明，miR-200c 可能是 OSCC 的一个潜在预后生物标志物。
