Persson U, Bick P H, Hammarströme L, Möller E, Smith C I
Scand J Immunol. 1978;8(4):291-301. doi: 10.1111/j.1365-3083.1978.tb00522.x.
Con A is known to activate T cells to proliferation and the development of effector cells. Conflicting reports have been published as to the need for accessory cells in the T-cell response induced by Con A. We have found that the proliferative response in purified mouse spleen T cells induced by various doses of Con A requires different culture conditions and helper cells. The Con-A-induced response induced by optimal and high concentrations of the ligand requires the presence of either serum or adherent cells obtained from the peritoneal cavity or the spleen. For induction of a proliferative response by low doses of Con A both serum and helper cells must be present. The T-cell response to suboptimal concentrations of Con A is further characterized by the fact that the presence of an Ia-positive cell is required. Removal of Ia-positive cells from purified T cells results in a loss of the response of remaining cells to low doses of Con A but has less effect on the response induced by higher concentrations of the activating ligand. The possibility that distinct subsets of T cells are responding to low and high concentrations of Con A will be discussed.
已知刀豆球蛋白A（Con A）可激活T细胞增殖并促使效应细胞发育。关于在Con A诱导的T细胞反应中是否需要辅助细胞，已有相互矛盾的报道发表。我们发现，不同剂量的Con A诱导纯化小鼠脾脏T细胞的增殖反应需要不同的培养条件和辅助细胞。由最佳和高浓度配体诱导的Con - A诱导反应需要存在血清或从腹腔或脾脏获得的贴壁细胞。对于低剂量Con A诱导增殖反应，血清和辅助细胞都必须存在。T细胞对次优浓度Con A的反应的进一步特征是需要Ia阳性细胞的存在。从纯化的T细胞中去除Ia阳性细胞会导致剩余细胞对低剂量Con A的反应丧失，但对高浓度激活配体诱导的反应影响较小。将讨论不同亚群的T细胞对低浓度和高浓度Con A作出反应的可能性。
