Shchubinskiĭ G Z
Biull Eksp Biol Med. 1983 Feb;95(2):64-6.
Incubation of human peripheral blood lymphocytes with concanavalin A (Con A), in a low suboptimal dose (0.5 microgram/ml), results in formation of the cells that inhibit proliferation of autologous cells in cultures activated with optimal but not with suboptimal dose of the mitogen. Nevertheless, 50 micrograms/ml Con A-activated cells efficiently suppress proliferation everywhere. Cell preincubation during 18 h before Con A activation leads to a reduction of lymphocyte responses to the mitogen in cultures reactivated with 5 micrograms/ml Con A in a mixture with autologous lymphocytes, containing no mitogen. Activation of T-T helper cells providing suppressor T cells differentiation seems to take place in the presence of a low suboptimal dose of Con A. Besides, 0.5 microgram/ml Con A prevents the preincubation-induced elimination of some lymphocytes responding to an optimal dose of Con A and autologous lymphocytes.
用人外周血淋巴细胞与低亚最佳剂量（0.5微克/毫升）的刀豆球蛋白A（Con A）孵育，会导致形成一些细胞，这些细胞在以最佳剂量而非亚最佳剂量的丝裂原激活的培养物中抑制自体细胞的增殖。然而，50微克/毫升Con A激活的细胞在各处均能有效抑制增殖。在Con A激活前18小时进行细胞预孵育，会导致在用5微克/毫升Con A与不含丝裂原的自体淋巴细胞混合重新激活的培养物中，淋巴细胞对丝裂原的反应降低。提供抑制性T细胞分化的T - T辅助细胞的激活似乎在低亚最佳剂量的Con A存在下发生。此外，0.5微克/毫升Con A可防止预孵育诱导的对最佳剂量Con A有反应的一些淋巴细胞和自体淋巴细胞的消除。
