Ford G E
Aust J Biol Sci. 1986;39(1):31-6.
Electrophoretic variants of enzymes from 100 individual macroscopic sarcocysts from sheep carcasses were examined to see if they could serve as genetic markers for the identification of ovine sarcosporidia. Of the 31 enzymes screened, 12 that represented single genetic loci were tested. There were two patterns of isoenzyme mobility, which differed at 7 out of 12 of the loci being studied, and corresponded to enzymes from either 'fat' or 'thin' cysts, indicating that these two sarcocyst types may be considered quite different species. In a parallel study, extracts of microscopic sarcocysts digested from sheep heart muscle were compared with those from macroscopic sarcocysts and found to have a distinct electrophoretic isoenzyme profile for each of four loci.
对来自羊尸体的100个单个宏观肉孢子虫的酶的电泳变体进行了检测，以确定它们是否可作为鉴定绵羊肉孢子虫的遗传标记。在所筛选的31种酶中，对代表单个基因座的12种酶进行了测试。有两种同工酶迁移模式，在所研究的12个基因座中有7个不同，分别对应于“肥”或“瘦”囊肿的酶，这表明这两种肉孢子虫类型可能被视为完全不同的物种。在一项平行研究中，将从羊心肌中消化得到的微观肉孢子虫提取物与宏观肉孢子虫的提取物进行比较，发现四个基因座中的每一个都有独特的电泳同工酶谱。
