Welch-Jernigan Rebecca J, Abrahamse Evan, Stoll Barbara, Smith O'Brian, Wierenga Peter A, van de Heijning Bert J M, Renes Ingrid B, Burrin Douglas G
Center for Comparative Medicine, Baylor College of Medicine, Houston, TX.
Danone Nutricia Research, Utrecht, the Netherlands.
Curr Dev Nutr. 2018 Dec 19;3(4):nzy102. doi: 10.1093/cdn/nzy102. eCollection 2019 Apr.
Multiple studies have indicated that formula-fed infants show a different growth trajectory compared with breastfed infants. The observed growth rates are suggested to be linked to higher postprandial levels of branched chain amino acids (BCAAs) and insulin related to differences in protein quality.
We evaluated the effects of milk protein denaturation and milk protein composition on postprandial plasma and hormone concentrations.
Neonatal piglets were bolus-fed randomly, in an incomplete crossover design, 2 of 3 milk protein solutions: native whey protein isolate (NWPI), denatured whey protein isolate (DWPI), or protein base ingredient, comprising whey and casein (PBI). Postprandial plasma amino acids (AAs), insulin, glucagon-like peptide 1, glucose, and paracetamol concentrations were assayed. Plasma responses were fitted with a model of first-order absorption with linear elimination.
DWPI (91% denatured protein) compared with NWPI (91% native protein) showed lower essential amino acids (EAAs) (∼10%) and BCAA (13-19%) concentrations in the first 30-60 min. However, total amino acid (TAA) concentration per time-point and area under the curve (AUC), as well as EAA and BCAA AUC were not different. PBI induced a ∼30% lower postprandial insulin spike than NWPI, yet plasma TAA concentration at several time-points and AUC was higher in PBI than in NWPI. The TAA rate constant for absorption ( ) was twofold higher in PBI than in NWPI. Plasma BCAA levels from 60 to 180 min and AUC were higher in PBI than in NWPI. Plasma EAA concentrations and AUCs in PBI and NWPI were not different.
Denaturation of WPI had a minimal effect on postprandial plasma AA concentration. The differences between PBI and NWPI were partly explained by the difference in AA composition, but more likely differences in protein digestion and absorption kinetics. We conclude that modifying protein composition, but not denaturation, of milk protein solutions impacts the postprandial amino acid availability in neonatal piglets.
多项研究表明,与母乳喂养的婴儿相比,配方奶喂养的婴儿呈现出不同的生长轨迹。观察到的生长速率被认为与餐后较高水平的支链氨基酸(BCAAs)和胰岛素有关,这与蛋白质质量的差异有关。
我们评估了乳蛋白变性和乳蛋白组成对餐后血浆和激素浓度的影响。
采用不完全交叉设计,将新生仔猪随机大剂量喂食三种乳蛋白溶液中的两种:天然乳清蛋白分离物(NWPI)、变性乳清蛋白分离物(DWPI)或包含乳清和酪蛋白的蛋白质基础成分(PBI)。测定餐后血浆氨基酸(AAs)、胰岛素、胰高血糖素样肽1、葡萄糖和对乙酰氨基酚的浓度。血浆反应采用一级吸收线性消除模型拟合。
与NWPI(91%天然蛋白)相比,DWPI(91%变性蛋白)在最初30 - 60分钟内的必需氨基酸(EAAs)浓度(约10%)和BCAA浓度(13 - 19%)较低。然而,每个时间点的总氨基酸(TAA)浓度和曲线下面积(AUC),以及EAA和BCAA的AUC并无差异。PBI诱导的餐后胰岛素峰值比NWPI低约30%,但在几个时间点PBI的血浆TAA浓度和AUC高于NWPI。PBI的TAA吸收速率常数( )比NWPI高两倍。PBI在60至180分钟的血浆BCAA水平和AUC高于NWPI。PBI和NWPI的血浆EAA浓度和AUC没有差异。
WPI的变性对餐后血浆AA浓度影响极小。PBI和NWPI之间的差异部分由AA组成的差异解释,但更可能是蛋白质消化和吸收动力学的差异。我们得出结论,改变乳蛋白溶液的蛋白质组成而非变性会影响新生仔猪餐后氨基酸的可利用性。