Suppr超能文献

新生仔猪乳蛋白混合物的餐后氨基酸动力学受组成影响,但不受变性影响。

Postprandial Amino Acid Kinetics of Milk Protein Mixtures are Affected by Composition, But Not Denaturation, in Neonatal Piglets.

作者信息

Welch-Jernigan Rebecca J, Abrahamse Evan, Stoll Barbara, Smith O'Brian, Wierenga Peter A, van de Heijning Bert J M, Renes Ingrid B, Burrin Douglas G

机构信息

Center for Comparative Medicine, Baylor College of Medicine, Houston, TX.

Danone Nutricia Research, Utrecht, the Netherlands.

出版信息

Curr Dev Nutr. 2018 Dec 19;3(4):nzy102. doi: 10.1093/cdn/nzy102. eCollection 2019 Apr.

Abstract

BACKGROUND

Multiple studies have indicated that formula-fed infants show a different growth trajectory compared with breastfed infants. The observed growth rates are suggested to be linked to higher postprandial levels of branched chain amino acids (BCAAs) and insulin related to differences in protein quality.

OBJECTIVE

We evaluated the effects of milk protein denaturation and milk protein composition on postprandial plasma and hormone concentrations.

METHODS

Neonatal piglets were bolus-fed randomly, in an incomplete crossover design, 2 of 3 milk protein solutions: native whey protein isolate (NWPI), denatured whey protein isolate (DWPI), or protein base ingredient, comprising whey and casein (PBI). Postprandial plasma amino acids (AAs), insulin, glucagon-like peptide 1, glucose, and paracetamol concentrations were assayed. Plasma responses were fitted with a model of first-order absorption with linear elimination.

RESULTS

DWPI (91% denatured protein) compared with NWPI (91% native protein) showed lower essential amino acids (EAAs) (∼10%) and BCAA (13-19%) concentrations in the first 30-60 min. However, total amino acid (TAA) concentration per time-point and area under the curve (AUC), as well as EAA and BCAA AUC were not different. PBI induced a ∼30% lower postprandial insulin spike than NWPI, yet plasma TAA concentration at several time-points and AUC was higher in PBI than in NWPI. The TAA rate constant for absorption ( ) was twofold higher in PBI than in NWPI. Plasma BCAA levels from 60 to 180 min and AUC were higher in PBI than in NWPI. Plasma EAA concentrations and AUCs in PBI and NWPI were not different.

CONCLUSIONS

Denaturation of WPI had a minimal effect on postprandial plasma AA concentration. The differences between PBI and NWPI were partly explained by the difference in AA composition, but more likely differences in protein digestion and absorption kinetics. We conclude that modifying protein composition, but not denaturation, of milk protein solutions impacts the postprandial amino acid availability in neonatal piglets.

摘要

背景

多项研究表明,与母乳喂养的婴儿相比,配方奶喂养的婴儿呈现出不同的生长轨迹。观察到的生长速率被认为与餐后较高水平的支链氨基酸(BCAAs)和胰岛素有关,这与蛋白质质量的差异有关。

目的

我们评估了乳蛋白变性和乳蛋白组成对餐后血浆和激素浓度的影响。

方法

采用不完全交叉设计,将新生仔猪随机大剂量喂食三种乳蛋白溶液中的两种:天然乳清蛋白分离物(NWPI)、变性乳清蛋白分离物(DWPI)或包含乳清和酪蛋白的蛋白质基础成分(PBI)。测定餐后血浆氨基酸(AAs)、胰岛素、胰高血糖素样肽1、葡萄糖和对乙酰氨基酚的浓度。血浆反应采用一级吸收线性消除模型拟合。

结果

与NWPI(91%天然蛋白)相比,DWPI(91%变性蛋白)在最初30 - 60分钟内的必需氨基酸(EAAs)浓度(约10%)和BCAA浓度(13 - 19%)较低。然而,每个时间点的总氨基酸(TAA)浓度和曲线下面积(AUC),以及EAA和BCAA的AUC并无差异。PBI诱导的餐后胰岛素峰值比NWPI低约30%,但在几个时间点PBI的血浆TAA浓度和AUC高于NWPI。PBI的TAA吸收速率常数( )比NWPI高两倍。PBI在60至180分钟的血浆BCAA水平和AUC高于NWPI。PBI和NWPI的血浆EAA浓度和AUC没有差异。

结论

WPI的变性对餐后血浆AA浓度影响极小。PBI和NWPI之间的差异部分由AA组成的差异解释,但更可能是蛋白质消化和吸收动力学的差异。我们得出结论,改变乳蛋白溶液的蛋白质组成而非变性会影响新生仔猪餐后氨基酸的可利用性。

相似文献

1
Postprandial Amino Acid Kinetics of Milk Protein Mixtures are Affected by Composition, But Not Denaturation, in Neonatal Piglets.
Curr Dev Nutr. 2018 Dec 19;3(4):nzy102. doi: 10.1093/cdn/nzy102. eCollection 2019 Apr.
3
Protein Ingredient Quality within Infant Formulas Impacts Plasma Amino Acid Concentrations in Neonatal Minipiglets.
J Nutr. 2024 Jul;154(7):2029-2041. doi: 10.1016/j.tjnut.2024.05.009. Epub 2024 May 25.
5
Postprandial Plasma Amino Acid Responses Between Standard Whey Protein Isolate and Whey Protein Isolate Plus Novel Technology.
Nutr Metab Insights. 2019 Feb 17;12:1178638819827970. doi: 10.1177/1178638819827970. eCollection 2019.
9
Proteins and Amino Acids Treated with Atmospheric Plasma Show Significantly Increased Bioavailability in Humans.
Nutr Metab Insights. 2020 Sep 28;13:1178638820949239. doi: 10.1177/1178638820949239. eCollection 2020.
10
Postprandial plasma amino acids in preterm infants: influence of the protein source.
Acta Paediatr. 1999 Aug;88(8):885-9. doi: 10.1080/08035259950168838.

引用本文的文献

本文引用的文献

4
Breastfeeding and Complementary Feeding.
Dtsch Arztebl Int. 2016 Jun 24;113(25):435-44. doi: 10.3238/arztebl.2016.0435.
6
Controlling the Ratio between Native-Like, Non-Native-Like, and Aggregated β-Lactoglobulin after Heat Treatment.
J Agric Food Chem. 2016 Jun 1;64(21):4362-70. doi: 10.1021/acs.jafc.6b00816. Epub 2016 May 23.
7
Early Programming by Protein Intake: The Effect of Protein on Adiposity Development and the Growth and Functionality of Vital Organs.
Nutr Metab Insights. 2016 Mar 20;8(Suppl 1):49-56. doi: 10.4137/NMI.S29525. eCollection 2015.
8
High protein intake in young children and increased weight gain and obesity risk.
Am J Clin Nutr. 2016 Feb;103(2):303-4. doi: 10.3945/ajcn.115.128009. Epub 2016 Jan 20.

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验