Pattnaik Subhaswaraj, Barik Subhashree, Muralitharan Gangatharan, Busi Siddhardha
Department of Microbiology, School of Life Sciences, Pondicherry University, Puducherry-605 014, India.
Department of Microbiology, School of Life Sciences, Bharathidasan University, Tiruchirappali-620 024, India.
IET Nanobiotechnol. 2018 Dec;12(8):1056-1061. doi: 10.1049/iet-nbt.2018.5114.
is an opportunistic nosocomial pathogenic microorganism causing majority of acute hospital-acquired infections and poses a serious public health concern. The persistence of bacterial infection can be attributed to the highly synchronised cell-to-cell communication phenomenon, quorum sensing (QS) which regulates the expression of a number of virulence factors and biofilm formation which eventually imparts resistance to the conventional antimicrobial therapy. In this study, the anti-quorum sensing and anti-biofilm potential of ferulic acid encapsulated chitosan-tripolyphosphate nanoparticles (FANPs) was investigated against PAO1 and compared with native ferulic acid. Dynamic light scattering and transmission electron microscopic analysis confirmed the synthesis of FANPs with mean diameter of 215.55 nm. FANPs showed significant anti-quorum sensing activity by downregulating QS-regulated virulence factors. In addition, FANPs also significantly attenuate the swimming and swarming motility of PAO1. The anti-biofilm efficacy of FANPs as compared to native ferulic acid was established by light and confocal laser scanning microscopic analysis. The promising results of FANPs in attenuating QS highlighted the slow and sustained release of ferulic acid at the target sites with greater efficacy suggesting its application towards the development of anti-infective agents.
是一种机会性医院致病微生物，导致大多数急性医院获得性感染，引起严重的公共卫生问题。细菌感染的持续存在可归因于高度同步的细胞间通讯现象——群体感应（QS），它调节多种毒力因子的表达以及生物膜的形成，而生物膜最终会使细菌对传统抗菌疗法产生抗性。在本研究中，研究了阿魏酸包封的壳聚糖 - 三聚磷酸钠纳米颗粒（FANPs）对铜绿假单胞菌PAO1的群体感应抑制和抗生物膜潜力，并与天然阿魏酸进行了比较。动态光散射和透射电子显微镜分析证实了平均直径为215.55 nm的FANPs的合成。FANPs通过下调群体感应调节的毒力因子表现出显著的群体感应抑制活性。此外，FANPs还显著减弱了PAO1的游动和群集运动能力。通过光学和共聚焦激光扫描显微镜分析确定了FANPs与天然阿魏酸相比的抗生物膜功效。FANPs在减弱群体感应方面的良好结果突出了阿魏酸在靶位点的缓慢持续释放及其更高的功效，表明其在抗感染药物开发中的应用前景。
