MWCNTs-COOH 颗粒在激光辐照下对 CT26 成纤维细胞的光致敏效应。

Photosensitizer effects of MWCNTs-COOH particles on CT26 fibroblastic cells exposed to laser irradiation.

机构信息

a Medical Physics Department, Faculty of Medicine , Shahid Sadoughi University of Medical Sciences , Yazd , Iran.

b Department of Medical Physics, School of Medicine , Iran University of Medical Sciences , Tehran , Iran.

出版信息

Artif Cells Nanomed Biotechnol. 2019 Dec;47(1):1326-1334. doi: 10.1080/21691401.2019.1593997.

DOI:10.1080/21691401.2019.1593997

PMID:30964347

Abstract

In the current study, photosensitizer effect of carboxylate multiwalled carbon nanotubes (MWCNTs-COOH) on CT26 fibroblastic cells following near infrared (NIR) irradiation was quantized in photo-thermal therapy (PTT). Moreover, it was tried to achieve optimal dose of MWCNTs-COOH and laser exposure time. Characterizations of MWCNTs-COOH were scrutinized using scanner electron microscope (SEM), spectrophotometer, and particle size analyzer. The seeded CT26 cells were treated with nontoxic concentrations of MWCNTs-COOH and then irradiated. Finally, viability (%) of the CT26 cells was determined using MTT assay. The findings revealed that 10, 50, and 80 μg/mL of MWCNTs-COOH have remarkable photosensitizer effects on CT26 cancerous cell lines against NIR irradiation (2.5 W/cm). It was shown that using the 80 μg/mL concentration of MWCNTs-COOH against 60, 120, 180, 240, and 300 s of NIR irradiation and also, 10 and 50 μg/mL concentration of MWCNTs-COOH against 180, 240, and 300 s of NIR irradiation can lead to significant decrease in mean cell viability (%) by more than 50%. According to the obtained data, it seems that using the PPT with MWCNTs-COOH, as adjunct therapy in CT26 fibroblastic cells, can help to increase therapeutic ratio of main modalities of cancer treatment such as radiotherapy, chemotherapy, and surgery.

摘要

在本研究中，羧基化多壁碳纳米管（MWCNTs-COOH）在近红外（NIR）照射下对 CT26 成纤维细胞的光热治疗（PTT）效果进行了量化。此外，还尝试确定 MWCNTs-COOH 的最佳剂量和激光照射时间。使用扫描电子显微镜（SEM）、分光光度计和粒度分析仪对 MWCNTs-COOH 的特性进行了仔细研究。将接种的 CT26 细胞用无毒浓度的 MWCNTs-COOH 处理，然后进行照射。最后，使用 MTT 测定法测定 CT26 细胞的活力（%）。结果表明，10、50 和 80μg/mL 的 MWCNTs-COOH 对 NIR 照射下的 CT26 癌细胞系具有显著的光敏化作用（2.5 W/cm）。结果表明，使用 80μg/mL 浓度的 MWCNTs-COOH 对 60、120、180、240 和 300s 的 NIR 照射以及 10 和 50μg/mL 浓度的 MWCNTs-COOH 对 180、240 和 300s 的 NIR 照射，可使平均细胞活力（%）显著降低超过 50%。根据获得的数据，似乎可以使用 MWCNTs-COOH 进行 PTT，作为 CT26 成纤维细胞的辅助治疗，以帮助提高放射治疗、化学疗法和手术等癌症主要治疗方式的治疗比率。