Department of Physiology, Institute for Biological Research "Sinisa Stankovic", University of Belgrade , Belgrade , Serbia.
IVF Department, The Clinic of Gynecology and Obstetrics "Narodni front" , Belgrade , Serbia.
Syst Biol Reprod Med. 2019 Oct;65(5):343-349. doi: 10.1080/19396368.2019.1600171. Epub 2019 Apr 9.
Protein expression/activity of antioxidative defense enzymes (AD) in seminal plasma of fertile men might be used as biomarkers of male fertility status. To test this concept, the present study examined the semen parameters of males among 14 normal idiopathic (normozoospermia) and 84 subnormal (teratozoospermia, oligoteratozoospermia, oligoasthenoteratozoospermia) infertile individuals. We investigated levels of protein expression/activity of Cu, Zn superoxide dismutase (CuZnSOD), manganese superoxide dismutase (MnSOD), catalase and glutathione peroxidase (GSH-Px), their association with functional sperm parameters, as well as their potential to serve as biomarkers of specific sperm pathologies. Although the activity of CuZnSOD and protein expression of catalase were significantly correlated with several sperm parameters, underlying their potential role in etiology of various sperm abnormalities, investigation of their potential usefulness as a biomarker of semen quality showed that these AD enzymes could not distinguish subtle differences between various sperm pathologies. In contrast, GSH-Px activity was decreased in all groups with sperm pathologies and was a very good indicator of aberrations in functional sperm parameters, explaining up to 94.6% of infertility cases where functional sperm parameters were affected. Therefore, assessment of GSH-Px activity showed the potential to discriminate between infertile males with normal and subnormal semen characteristics and may prove useful in the evaluation of male (in)fertility. AD: antioxidative defense; Cu, Zn SOD: copper, zinc superoxide dismutase; GSH-Px: glutathione peroxidase; MnSOD: manganese superoxide dismutase; NS: normospermia; OATS: oligoasthenoteratozoospermia; OTS: oligoteratozoospermia; ROC: receiver operating characteristic; ROS: reactive oxygen species; TS: teratozoospermia; WHO: world health organization.
精液中抗氧化防御酶（AD）的蛋白表达/活性可作为男性生育力状况的生物标志物。为了验证这一概念，本研究检查了 14 名正常特发性（正常精子症）和 84 名异常（畸形精子症、少精子症、少弱精子症、弱精子症）不育男性的精液参数。我们研究了铜锌超氧化物歧化酶（CuZnSOD）、锰超氧化物歧化酶（MnSOD）、过氧化氢酶和谷胱甘肽过氧化物酶（GSH-Px）的蛋白表达/活性水平及其与功能精子参数的相关性，以及它们作为特定精子病变生物标志物的潜力。尽管 CuZnSOD 活性和过氧化氢酶蛋白表达与几个精子参数显著相关，表明它们在各种精子异常的病因学中具有潜在作用，但研究其作为精液质量生物标志物的潜在用途表明，这些 AD 酶不能区分各种精子病变之间的细微差异。相比之下，所有精子病变组的 GSH-Px 活性均降低，是功能精子参数异常的非常好的指标，可解释高达 94.6%的功能精子参数受影响的不育病例。因此，GSH-Px 活性评估有可能区分具有正常和异常精液特征的不育男性，并且可能有助于评估男性生育能力。AD：抗氧化防御；Cu，Zn SOD：铜，锌超氧化物歧化酶；GSH-Px：谷胱甘肽过氧化物酶；MnSOD：锰超氧化物歧化酶；NS：正常精子症；OATS：少弱畸形精子症；OTS：少精子症；ROC：接收者操作特征；ROS：活性氧；TS：畸形精子症；WHO：世界卫生组织。
