Llavanera Marc, Delgado-Bermúdez Ariadna, Mateo-Otero Yentel, Padilla Lorena, Romeu Xavier, Roca Jordi, Barranco Isabel, Yeste Marc
Unit of Cell Biology, Department of Biology, Faculty of Sciences, University of Girona, ES-17003 Girona, Spain.
Biotechnology of Animal and Human Reproduction (TechnoSperm), Institute of Food and Agricultural Technology, University of Girona, ES-17003 Girona, Spain.
Antioxidants (Basel). 2020 Aug 12;9(8):741. doi: 10.3390/antiox9080741.
Glutathione S-transferases Mu 3 (GSTM3) is an essential antioxidant enzyme whose presence in sperm has recently been related to sperm cryotolerance, quality and fertility. However, its role in seminal plasma (SP) as a predictor of the same sperm parameters has never been investigated. Herein, cell biology and proteomic approaches were performed to explore the presence, origin and role of SP-GSTM3 as a sperm quality and in vivo fertility biomarker. GSTM3 in SP was quantified using a commercial Enzyme-Linked Immunosorbent Assay (ELISA) kit specific for , whereas the presence of GSTM3 in testis, epididymis and accessory sex glands was assessed through immunoblotting analysis. Sperm quality and functionality parameters were evaluated in semen samples at 0 and 72 h of liquid-storage, whereas fertility parameters were recorded over a 12-months as farrowing rate and litter size. The presence and concentration of GSTM3 in SP was established for the first time in mammalian species, predominantly synthesized in the epididymis. The present study also evidenced a relationship between SP-GSTM3 and sperm morphology and suggested it is involved in epididymal maturation rather than in ejaculated sperm physiology. Finally, the data reported herein ruled out the role of this antioxidant enzyme as a quality and in vivo fertility biomarker of pig sperm.
谷胱甘肽S-转移酶Mu 3（GSTM3）是一种重要的抗氧化酶，其在精子中的存在最近被认为与精子冷冻耐受性、质量和生育能力有关。然而，其在精浆（SP）中作为相同精子参数预测指标的作用从未被研究过。在此，采用细胞生物学和蛋白质组学方法来探究精浆GSTM3作为精子质量和体内生育能力生物标志物的存在、来源及作用。使用针对GSTM3的商业酶联免疫吸附测定（ELISA）试剂盒对精浆中的GSTM3进行定量，而通过免疫印迹分析评估睾丸、附睾和附属性腺中GSTM3的存在情况。在精液样本储存0小时和72小时时评估精子质量和功能参数，而生育参数记录12个月，包括产仔率和窝产仔数。首次在哺乳动物物种中确定了精浆中GSTM3的存在和浓度，其主要在附睾中合成。本研究还证明了精浆GSTM3与精子形态之间的关系，并表明它参与附睾成熟而非射精后精子的生理过程。最后，本文报道的数据排除了这种抗氧化酶作为猪精子质量和体内生育能力生物标志物的作用。
