Department of Dental Materials and Prosthodontics, UNESP - Univ. Estadual Paulista, Araraquara School of Dentistry, Araraquara, Brazil.
Department of Dentistry, Universidade de Ribeirão Preto, UNAERP, Ribeirão Preto, Brazil.
Arch Oral Biol. 2019 Jun;102:48-54. doi: 10.1016/j.archoralbio.2019.03.025. Epub 2019 Mar 29.
Different strategies, such as modifications on the implant abutments surface have been proposed to accelerate and improve the formation of the biological seal (BS). The aim of this study was to characterize a titanium (Ti) surface impregnated with epidermal growth factor (EGF) and to assess its influence on the metabolism and adhesion of oral mucosal cells.
Ti discs were coated with EGF (100 nM) conjugated with a fluorophore and analyzed by fluorescence microscopy. The surface roughness analysis (Ra) of the EGF-coated Ti was performed by confocal microscopy. The EGF released in the wet environment was determined at 0, 24, 48 and 72 h by fluorimetric quantification. For assessment of the biological effects of EGF-coated Ti, gingival fibroblasts were seeded (5 × 10 cells) onto the substrate coated or not with this growth factor. After 24 h, cell adhesion and viability were evaluated by ANOVA and Tukey tests, α = .05.
Immediate release of EGF as well as its incorporation by fibroblasts within 1 h after cells were seeded was observed. EGF-coated Ti discs presented significantly enhance surface roughness. Increased cell viability was observed on the EGF-coated discs.
EGF applied to Ti discs stimulated the adhesion and metabolism of gingival fibroblasts and could be considered as an interesting alternative for improving the BS.
为了加速和改善生物密封（BS）的形成，已经提出了不同的策略，例如对种植体基台表面进行修改。本研究的目的是对表皮生长因子（EGF）浸渍的钛（Ti）表面进行特征描述，并评估其对口腔黏膜细胞代谢和黏附的影响。
将 EGF（100 nM）与荧光团缀合涂覆在 Ti 盘上，并通过荧光显微镜进行分析。通过共聚焦显微镜对 EGF 涂覆 Ti 的表面粗糙度分析（Ra）进行了分析。通过荧光定量法在 0、24、48 和 72 h 时测定湿环境中释放的 EGF。为了评估 EGF 涂覆 Ti 的生物学效应，将牙龈成纤维细胞（5×10 个细胞）接种到涂覆或未涂覆该生长因子的底物上。24 h 后，通过方差分析和 Tukey 检验评估细胞黏附和活力，α=0.05。
观察到 EGF 的即刻释放以及细胞接种后 1 h 内成纤维细胞对其的摄取。EGF 涂覆 Ti 盘的表面粗糙度显著增加。在 EGF 涂覆的培养皿上观察到细胞活力增加。
EGF 施加到 Ti 盘上刺激了牙龈成纤维细胞的黏附和代谢，可被视为改善 BS 的一种有前途的方法。
