Department of Dental Materials and Prosthodontics, Araraquara School of Dentistry, São Paulo State University (UNESP), Araraquara, Brazil.
Department of Dentistry, Universidade de Ribeirão Preto, UNAERP, Ribeirão Preto, SP, Brazil.
Clin Oral Investig. 2021 Oct;25(10):5775-5784. doi: 10.1007/s00784-021-03880-1. Epub 2021 Apr 14.
To assess the effects of epidermal growth factor (EGF)-coated titanium (Ti) discs on the adhesion and metabolism of keratinocytes and gingival fibroblasts exposed to nitrogen-containing bisphosphonates.
Keratinocytes and fibroblasts were seeded (1 × 10 cells/disc) on Ti discs coated with EGF (100 nM). After 24 h, cells were exposed or not to sodium alendronate (SA) or zoledronic acid (ZA) at different concentrations (0 = control, 0.5, 1, or 5 μM) for 48 h. Cell adhesion to the substrates was evaluated by fluorescence microscopy. Cell viability (alamarBlue, n = 6) and synthesis of vascular endothelial growth factor (VEGF), matrix metalloproteinase-2 (MMP-2), and keratinocytes growth factor (KGF) (ELISA, n = 6) were assessed. Data were statistically analyzed by one-way ANOVA and Tukey tests (α = 0.05).
Higher cell adhesion rate was observed when keratinocytes and fibroblasts were seeded onto EGF-coated discs in comparison to uncoated discs. ZA treatment hindered the adhesion of both cell lines on the Ti discs as well as reduced the viability and synthesis of VEGF, KGF and MMP-2 by cells (p < 0.05). SA treatment did not affect cell viability, but interfered negatively on the adhesion and synthesis of EGF and KGF by the cells (p < 0.05). EGF-coated surface increased cell viability and synthesis of growth factors as well as downregulated the synthesis of MMP-2 in comparison to control (p < 0.05).
EGF applied on Ti surface improves the biological responses of oral mucosa cells exposed to SA and ZA.
EGF-coating on titanium may be a suitable strategy to improve oral mucosa cellular events related to biological sealing, especially for patients under bisphosphonate therapy.
评估表皮生长因子（EGF）涂覆钛（Ti）盘对暴露于含氮双膦酸盐的角质形成细胞和牙龈成纤维细胞的黏附和代谢的影响。
将角质形成细胞和成纤维细胞（1×10 个细胞/盘）接种在 EGF（100 nM）涂覆的 Ti 盘上。24 h 后，将细胞暴露或不暴露于不同浓度的阿仑膦酸钠（SA）或唑来膦酸（ZA）（0=对照，0.5、1 或 5 μM）48 h。通过荧光显微镜评估细胞在基质上的黏附。通过 alamarBlue 评估细胞活力（n=6），通过 ELISA 评估血管内皮生长因子（VEGF）、基质金属蛋白酶-2（MMP-2）和角质细胞生长因子（KGF）的合成（n=6）。数据采用单因素方差分析和 Tukey 检验进行统计学分析（α=0.05）。
与未涂覆盘相比，当角质形成细胞和成纤维细胞接种在 EGF 涂覆的盘上时，观察到更高的细胞黏附率。ZA 处理不仅阻碍了两种细胞系在 Ti 盘上的黏附，还降低了细胞 VEGF、KGF 和 MMP-2 的活力和合成（p<0.05）。SA 处理不影响细胞活力，但会对细胞的 EGF 和 KGF 黏附和合成产生负面影响（p<0.05）。与对照相比，EGF 涂覆表面提高了细胞活力和生长因子的合成，并下调了 MMP-2 的合成（p<0.05）。
EGF 施加在 Ti 表面上可改善暴露于 SA 和 ZA 的口腔黏膜细胞的生物学反应。
EGF 涂覆在钛上可能是改善与生物密封相关的口腔黏膜细胞事件的一种合适策略，特别是对于接受双膦酸盐治疗的患者。
