Department of Applied Chemistry, China Agricultural University, Yuanmingyuan West Road 2, Beijing, 100193, China; Beijing Advanced Innovation Center for Food Nutrition and Human Health, Yuanmingyuan West Road 2, Beijing, 100193, China.
Department of Applied Chemistry, China Agricultural University, Yuanmingyuan West Road 2, Beijing, 100193, China.
Chemosphere. 2019 Jul;226:817-824. doi: 10.1016/j.chemosphere.2019.04.007. Epub 2019 Apr 3.
Reptiles, the most diverse taxon of terrestrial vertebrates, might be particularly vulnerable to soil pollution. Reptiles especially lizards have been rarely evaluated in ecotoxicological studies, and there is a very limited report for effects of soil pesticide contaminants on lizards. In this study, male and female lizards (Eremias argus) were exposed to Glufosinate-ammonium (GLA) and l- Glufosinate-ammonium (L-GLA) for 60 days. Slower sprint speed, higher frequency of turning back and reduced brain index were observed in treatment groups. The accumulation of GLA in the brain of lizard was higher than that of L-GLA. Moreover, the activities of neurotoxicity-related enzymes and biomarkers of oxidative stress were also investigated. In summary, the neurotoxic effects of lizards have been observed after exposure to GLA and L-GLA. Based on the result of the Integrated Biomarker Response (IBR), males were more sensitive to contaminants than females. On the other hand, the neurotoxic pathways by GLA and L-GLA triggered were slightly different: GLA mainly acted on glutamine synthetase (GS), acetylcholinesterase (AchE) and Catalase (CAT) and L-GLA aimed at AchE, Na/K-ATPase, Superoxide dismutase (SOD) and Malondialdehyde (MDA). In summary, the accumulation of GLA and L-GLA in lizard's brain induced neurotoxicity by altering the levels of enzymes related to nervous system and antioxidant activity and further resulted in the decrease of brain index and locomotor performance.
爬行动物是陆地脊椎动物中最多样化的分类群，可能特别容易受到土壤污染的影响。爬行动物，特别是蜥蜴，在生态毒理学研究中很少被评估，而且关于土壤农药污染物对蜥蜴的影响的报告非常有限。在这项研究中，雄性和雌性蜥蜴（Eremias argus）被暴露于草铵膦（GLA）和 L-草铵膦（L-GLA）中 60 天。在处理组中观察到冲刺速度较慢、转身频率较高和大脑指数降低。蜥蜴大脑中 GLA 的积累量高于 L-GLA。此外，还研究了与神经毒性相关的酶和氧化应激生物标志物的活性。总之，在暴露于 GLA 和 L-GLA 后，蜥蜴的神经毒性已经被观察到。基于综合生物标志物反应（IBR）的结果，雄性比雌性对污染物更敏感。另一方面，GLA 和 L-GLA 引发的神经毒性途径略有不同：GLA 主要作用于谷氨酰胺合成酶（GS）、乙酰胆碱酯酶（AchE）和过氧化氢酶（CAT），而 L-GLA 则针对 AchE、Na/K-ATPase、超氧化物歧化酶（SOD）和丙二醛（MDA）。总之，GLA 和 L-GLA 在蜥蜴大脑中的积累通过改变与神经系统和抗氧化活性相关的酶的水平，导致大脑指数和运动表现下降，从而引起神经毒性。
