Department of Applied Chemistry, China Agricultural University, Yuanmingyuan West Road 2, Beijing 100193, China; Beijing Advanced Innovation Center for Food Nutrition and Human Health, Yuanmingyuan West Road 2, Beijing 100193, China.
Department of Applied Chemistry, China Agricultural University, Yuanmingyuan West Road 2, Beijing 100193, China.
Environ Pollut. 2020 Feb;257:113564. doi: 10.1016/j.envpol.2019.113564. Epub 2019 Nov 6.
In the context of global warming, an important issue is that many pesticides become more toxic, putting non-target organisms at higher risk of pesticide exposure. Eremias argus (a native Chinese lizard) was selected as animal model in this study. As a kind of poikilothermic vertebrate, E.argus is sensitive to temperature change. The experimental design [(with or without L-Glufosinate-ammonium (L-GLA) pollution × two temperatures (25 and 30 °C)] was used in this study for 90 days to identify the chronic effects of the pesticide-temperature interaction on the lizards' neuroendocrine-regulated reproduction. Survival rate, body weight, clutch characteristics, testicular histopathology, the content of neurotransmitters and related enzyme activity, the level of sex steroid, the expression of Heat shock protein 70 (HSP70), antioxidant system, the accumulation and degradation of L-GLA were examined. Results showed that L-GLA disrupt reproduction of lizards through hypothalamus-pituitary-gonad (HPG) axis. In addition, temperature can not only change the environmental behavior of pesticides, but also alter the physiological characteristics of lizards. Thus, our results emphasized that temperature is an essential abiotic factor that should not be overlooked in ecotoxicological studies.
在全球变暖的背景下，一个重要的问题是，许多杀虫剂变得毒性更大，使非目标生物面临更高的接触杀虫剂的风险。本研究选择中华鳖蜥（一种中国本土蜥蜴）作为动物模型。作为一种变温脊椎动物，中华鳖蜥对温度变化敏感。本研究采用实验设计[（有或没有 L-草铵膦铵（L-GLA）污染×两个温度（25 和 30°C）]，研究 90 天，以确定农药-温度相互作用对蜥蜴神经内分泌调节生殖的慢性影响。存活率、体重、卵窝特征、睾丸组织病理学、神经递质含量及相关酶活性、性类固醇水平、热休克蛋白 70（HSP70）的表达、抗氧化系统、L-GLA 的积累和降解均进行了检测。结果表明，L-GLA 通过下丘脑-垂体-性腺（HPG）轴干扰蜥蜴的繁殖。此外，温度不仅可以改变杀虫剂的环境行为，还可以改变蜥蜴的生理特征。因此，我们的研究结果强调，温度是生态毒理学研究中不可忽视的重要非生物因素。
