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用于筛查原料乳中抗生素的Delvotest P多板检测法的实际评估

A Practical Evaluation of the Delvotest P Multi Plate Test in Screening Raw Milk for Antibiotics.

作者信息

Larocque Lyse, Neville G A

机构信息

Chemical Standards Division, Bureau of Drug Research, Health Protection Branch, Health and Welfare Canada, Tunney's Pasture, Ottawa, Canada K1A 0L2.

出版信息

J Food Prot. 1986 Nov;49(11):868-870. doi: 10.4315/0362-028X-49.11.868.

Abstract

The Delvotest P Multi plate test was evaluated by screening 100 milk samples for total antibiotic residues (penicillin G, streptomycin and neomycin). The samples were taken in conjunction with an antibiotic depletion study in milk derived from six cows treated with a multiple antibiotic, intramammary infusion product. Within the limits of sensitivity of the Delvotest, only penicillin G persisted in milk samples taken beyond 60 h, whereas in some samples, the other antibiotics appeared to be depleted as early as 48 h. More sensitive tests, however, detected neomycin ( Staphylococcus epidermidis , ATCC 12228) in 50% of samples taken at 60 h and streptomycin ( Bacillus subtilis , ATCC 6633) at 14.5 d after discontinuation of infusion.

摘要

通过对100份牛奶样本进行总抗生素残留(青霉素G、链霉素和新霉素)筛查,对德尔伏斯特P多板检测法进行了评估。这些样本是在一项针对六头用多种抗生素乳房内输注产品治疗的奶牛所产牛奶的抗生素清除研究中采集的。在德尔伏斯特检测法的灵敏度范围内,仅青霉素G在60小时后采集的牛奶样本中仍有残留,而在一些样本中,其他抗生素早在48小时就似乎已被清除。然而,更灵敏的检测法在输注停止后60小时采集的50%样本中检测到了新霉素(表皮葡萄球菌,ATCC 12228),在14.5天时检测到了链霉素(枯草芽孢杆菌,ATCC 6633)。

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