Klinken S P, Castilla M J, Thorgeirsson S S
Cancer Res. 1986 Dec;46(12 Pt 1):6246-9.
alpha-Difluoromethylornithine (DFMO) and methyl acetylene putrescine (MAP) are inhibitors of the rate limiting enzyme in polyamine synthesis, ornithine decarboxylase. We studied the effects of these compounds on the formation of retrovirus transformed erythroid colonies. DFMO was able to effectively reduce the number of transformed colonies at a concentration of 10(-3) M, whereas MAP achieved total inhibition at 10(-4) M. Putrescine, the product of ornithine decarboxylase, did not alter colony number by itself but it was able to overcome the inhibitory effects of both DFMO and MAP. Addition of DFMO at times after the initiation of culture decreased its effectiveness in reducing transformed colony numbers, while the converse was true for the erythroid stimulant, erythropoietin. We concluded from these data that DFMO and MAP probably diminished colony formation by inhibiting proliferation of the target cells for the retroviruses.
α-二氟甲基鸟氨酸（DFMO）和甲基乙炔腐胺（MAP）是多胺合成限速酶鸟氨酸脱羧酶的抑制剂。我们研究了这些化合物对逆转录病毒转化的红系集落形成的影响。DFMO在浓度为10^(-3) M时能够有效减少转化集落的数量，而MAP在10^(-4) M时实现了完全抑制。鸟氨酸脱羧酶的产物腐胺本身不会改变集落数量，但它能够克服DFMO和MAP的抑制作用。在培养开始后的不同时间添加DFMO会降低其减少转化集落数量的有效性，而对于红系刺激因子促红细胞生成素来说则相反。从这些数据我们得出结论，DFMO和MAP可能通过抑制逆转录病毒靶细胞的增殖来减少集落形成。
