Faculty and Graduate School of Fisheries Sciences, Hokkaido University, Sapporo, Hokkaido 060-0808, Japan.
School of Marine Bioscience, Kitasato University, Minato City, Tokyo 108-0072, Japan.
Mar Drugs. 2019 Apr 8;17(4):216. doi: 10.3390/md17040216.
A novel protein, soritesidine (SOR) with potent toxicity was isolated from the marine sponge sp. SOR exhibited wide range of toxicities over various organisms and cells including brine shrimp () larvae, sea hare () eggs, mice, and cultured mammalian cells. Toxicities of SOR were extraordinary potent. It killed mice at 5 ng/mouse after intracerebroventricular (i.c.v.) injection, and brine shrimp and at 0.34 µg/mL. Cytotoxicity for cultured mammalian cancer cell lines against HeLa and L1210 cells were determined to be 0.062 and 12.11 ng/mL, respectively. The SOR-containing fraction cleaved plasmid DNA in a metal ion dependent manner showing genotoxicity of SOR. Purified SOR exhibited molecular weight of 108.7 kDa in MALDI-TOF MS data and isoelectric point of approximately 4.5. -terminal amino acid sequence up to the 25th residue was determined by Edman degradation. Internal amino acid sequences for fifteen peptides isolated from the enzyme digest of SOR were also determined. None of those amino acid sequences showed similarity to existing proteins, suggesting that SOR is a new proteinous toxin.
一种新型蛋白 soritesidine（SOR）具有很强的毒性，从海洋海绵 sp. 中分离得到。SOR 对包括丰年虾（）幼虫、海兔（）卵、小鼠和培养的哺乳动物细胞在内的各种生物和细胞表现出广泛的毒性。SOR 的毒性非常强。它经脑室内（i.c.v.）注射后，以 5ng/小鼠的剂量杀死小鼠，以 0.34μg/mL 的剂量杀死丰年虾。对培养的哺乳动物癌细胞系 HeLa 和 L1210 的细胞毒性分别为 0.062 和 12.11ng/mL。含 SOR 的部分以金属离子依赖的方式切割质粒 DNA，显示出 SOR 的遗传毒性。纯化的 SOR 在 MALDI-TOF MS 数据中显示分子量为 108.7kDa，等电点约为 4.5。通过 Edman 降解确定了 SOR 的 25 个残基 N-末端氨基酸序列。还从 SOR 的酶解产物中分离出的十五个肽的内部氨基酸序列也被确定。这些氨基酸序列均与现有蛋白无相似性，提示 SOR 是一种新型蛋白毒素。
