Nagai Hiroshi, Oshiro Naomasa, Takuwa-Kuroda Kyoko, Iwanaga Setsuko, Nozaki Masatoshi, Nakajima Terumi
Suntory Institute for Bioorganic Research, 1-1-1 Wakayamadai, Shimamoto, Mishima, Osaka 618-8503, Japan.
Biochem Biophys Res Commun. 2002 Jun 21;294(4):760-3. doi: 10.1016/S0006-291X(02)00547-8.
The Okinawan sea anemone Phyllodiscus semoni is known to cause cases of severe stinging. We isolated P. semoni toxins 60A and 60B (PsTX-60A and PsTX-60B; ca. 60 kDa) as the major toxins from the isolated nematocysts of this species for the first time. PsTX-60A and PsTX-60B showed lethal toxicity to the shrimp Palaemon paucidence when administered via intraperitoneal injection (LD(50) values: 800-900 and 800 microg/kg, respectively) and hemolytic activity toward a 0.8% suspension of sheep red blood cells (ED(50) values: 600 and 300 ng/ml, respectively). Furthermore, we sequenced the cDNA encoding PsTX-60A. The deduced amino acid sequence of PsTX-60A did not show any similarity to previously reported proteins. The N-terminal amino acid sequence of PsTX-60B showed homology with that of PsTX-60A. These toxins represent a novel class of cytolytic proteinaceous toxins.
已知冲绳海葵Phyllodiscus semoni会导致严重的蜇伤病例。我们首次从该物种分离出的刺丝囊中分离出P. semoni毒素60A和60B（PsTX - 60A和PsTX - 60B；约60 kDa）作为主要毒素。通过腹腔注射给予时，PsTX - 60A和PsTX - 60B对虾Palaemon paucidence显示出致死毒性（LD(50)值：分别为800 - 900和800微克/千克），并且对0.8%的绵羊红细胞悬液具有溶血活性（ED(50)值：分别为600和300纳克/毫升）。此外，我们对编码PsTX - 60A的cDNA进行了测序。PsTX - 60A推导的氨基酸序列与先前报道的蛋白质没有任何相似性。PsTX - 60B的N端氨基酸序列与PsTX - 60A的同源。这些毒素代表了一类新型的细胞溶解性蛋白质毒素。
