Cryo-EM 结构的伴随病毒双联粒子。
Cryo-EM Structure of a Begomovirus Geminate Particle.
机构信息
State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China.
Guangxi Key Laboratory of Medicinal Resources Conservation and Genetic Improvement, Guangxi Botanical Garden of Medicinal Plants, Nanning 530023, China.
出版信息
Int J Mol Sci. 2019 Apr 8;20(7):1738. doi: 10.3390/ijms20071738.
Tobacco curly shoot virus, a monopartite begomovirus associated with betasatellite, causes serious leaf curl diseases on tomato and tobacco in China. Using single-particle cryo-electron microscopy, we determined the structure of tobacco curly shoot virus (TbCSV) particle at 3.57 Å resolution and confirmed the characteristic geminate architecture with single-strand DNA bound to each coat protein (CP). The CP⁻CP and DNA⁻CP interactions, arranged in a CP⁻DNA⁻CP pattern at the interface, were partially observed. This suggests the genomic DNA plays an important role in forming a stable interface during assembly of the geminate particle.
烟草曲叶病毒，一种与卫星 β 伴随的单分体伴随病毒，在中国引起番茄和烟草的严重卷叶病。通过单颗粒冷冻电镜，我们确定了烟草曲叶病毒（TbCSV）颗粒的结构，分辨率为 3.57 Å，并证实了具有与每个外壳蛋白（CP）结合的单链 DNA 的特征双联体结构。在界面处排列成 CP-DNA-CP 模式的 CP-CP 和 DNA-CP 相互作用被部分观察到。这表明基因组 DNA 在双联体颗粒组装过程中形成稳定界面中发挥重要作用。
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