Research Center for Clinical Virology, Tehran University of Medical Sciences, Tehran, Iran.
Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran.
Gene. 2019 Dec 15;720:144096. doi: 10.1016/j.gene.2019.144096. Epub 2019 Aug 30.
Biologically active materials and polymeric materials used in tissue engineering have been one of the most attractive research areas in the past decades, especially the use of easily accessible materials from the patients that reduces or eliminates any patient's immune response. In this study, electrospun nanofibrous scaffolds were fabricated by using polyvinyl-alcohol (PVA), chitosan and hydroxyapatite (HA) polymers and platelet-rich plasma (PRP) as a bioactive substance isolated from human blood. Fabricated scaffold's structure and cytotoxicity were evaluated using scanning electron microscope and MTT assays. Scaffolds osteoinductivity was investigated by osteogenic differentiation of the mesenchymal stem cells (MSCs) at the in vitro level and then its osteoconductivity was examined by implanting at the critical-sized rat calvarial defect. The in vitro results showed that scaffolds have a good structure and good biocompatibility. Alkaline phosphatase activity, calcium content and gene expression assays were also demonstrated that their highest amount was detected in MSCs-seeded PVA-chitosan-HA(PRP) scaffold. For this reason, this scaffold alone and along with the MSCs was implanted to the animal defects. The in vivo results demonstrated that in the animals implanted with PVA-chitosan-HA(PRP), the defect was repaired to a good extent, but in those animals that received MSCs-seeded PVA-chitosan-HA(PRP), the defects was almost filled. It can be concluded that, PVA-chitosan-HA(PRP) alone or when stem cells cultured on them, has a great potential to use as an effective bone implant.
在过去几十年中，用于组织工程的生物活性材料和聚合物材料一直是最具吸引力的研究领域之一，特别是使用患者体内易得的材料可以减少或消除任何患者的免疫反应。在这项研究中，通过使用聚乙烯醇（PVA）、壳聚糖和羟基磷灰石（HA）聚合物以及从人血中分离出的富血小板血浆（PRP）作为生物活性物质，制备了电纺纳米纤维支架。使用扫描电子显微镜和 MTT 测定法评估了制备的支架的结构和细胞毒性。通过间充质干细胞（MSCs）在体外的成骨分化研究了支架的成骨诱导能力，然后通过在大鼠临界颅骨缺损处植入来检查其成骨传导性。体外结果表明，支架具有良好的结构和良好的生物相容性。碱性磷酸酶活性、钙含量和基因表达测定也表明，在接种 PVA-壳聚糖-HA（PRP）支架的 MSCs 中检测到的数量最多。因此，单独使用这种支架以及与 MSCs 一起植入动物缺陷部位。体内结果表明，在植入 PVA-壳聚糖-HA（PRP）的动物中，缺陷得到了很好的修复，但在接受接种 PVA-壳聚糖-HA（PRP）的 MSCs 的动物中，缺陷几乎被填满。可以得出结论，PVA-壳聚糖-HA（PRP）单独使用或在其上培养干细胞时，具有作为有效骨植入物的巨大潜力。
