DiMario P J, Mahowald A P
Cell Tissue Res. 1986;246(1):103-8. doi: 10.1007/BF00219005.
The endocytosis of labeled vitellogenin by the developing oocytes of Drosophila melanogaster is pH dependent and inhibited in the presence of primary amines as determined by culturing whole ovaries in vitro. When the pH of the culture medium is adjusted to 6.8 or above, the vitellogenic oocytes sequester labeled vitellogenin synthesized by the follicle cells. The endocytosis of vitellogenin is shown autoradiographically by the accumulation of labeled yolk spheres within the oocytes. When the pH of the medium is reduced to 6.6 or below, the oocytes fail to sequester labeled vitellogenin, as demonstrated by an increase in immunoprecipitable vitellogenin in the culture medium and a concomitant reduction in the number of labeled yolk spheres within the oocytes. Vitellogenin endocytosis is also impaired by the addition of the primary amines methylamine or chloroquine to the culture medium. Monensin, a carboxylic ionophore, is shown to inhibit completely the secretion of labeled vitellogenin from the follicle cells.
通过体外培养完整卵巢确定，果蝇发育中的卵母细胞对标记卵黄原蛋白的内吞作用依赖于pH值，并且在伯胺存在的情况下受到抑制。当培养基的pH值调节至6.8或更高时，卵黄生成的卵母细胞会隔离由卵泡细胞合成的标记卵黄原蛋白。通过卵母细胞内标记卵黄球的积累，放射自显影显示了卵黄原蛋白的内吞作用。当培养基的pH值降至6.6或更低时，卵母细胞无法隔离标记卵黄原蛋白，培养基中可免疫沉淀的卵黄原蛋白增加以及卵母细胞内标记卵黄球数量随之减少证明了这一点。向培养基中添加伯胺甲胺或氯喹也会损害卵黄原蛋白的内吞作用。羧酸离子载体莫能菌素被证明可完全抑制卵泡细胞分泌标记卵黄原蛋白。
