Tanasescu Radu, Mettal Ute, Colom Adai, Roux Aurélien, Zumbuehl Andreas
Department of Chemistry, University of Fribourg, Chemin du Musée 9, 1700 Fribourg, Switzerland.
Department of Biochemistry, University of Geneva, 30, Quai Ernest-Ansermet, 1211 Geneva, Switzerland.
Polymers (Basel). 2018 Jan 9;10(1):54. doi: 10.3390/polym10010054.
Giant vesicles (GVs) are widely-used model systems for biological membranes. The formulation of these vesicles, however, can be problematic and artifacts, such as degraded molecules or left-over oil, may be present in the final liposomes. The rapid formulation of a high number of artifact-free vesicles of uniform size using standard laboratory equipment is, therefore, highly desirable. Here, the gentle hydration method of glass bead-supported thin lipid films has been enhanced by adding a vortexing step. This led to the formulation of a uniform population of giant vesicles. Batches of glass beads coated with different lipids can be combined to produce vesicles of hybrid lipid compositions. This method represents a stable approach to rapidly generate giant vesicles.
巨型囊泡（GVs）是用于生物膜的广泛使用的模型系统。然而，这些囊泡的制备可能存在问题，并且最终的脂质体中可能存在诸如降解分子或残留油等假象。因此，非常希望使用标准实验室设备快速制备大量无假象、大小均匀的囊泡。在这里，通过添加涡旋步骤改进了玻璃珠支撑的薄脂质膜的温和水合方法。这导致了均匀的巨型囊泡群体的形成。可以将涂覆有不同脂质的玻璃珠批次组合起来，以产生具有混合脂质组成的囊泡。该方法代表了一种快速生成巨型囊泡的稳定方法。
